Table 2

Flinders Island Spotted Fever Rickettsioses Caused by “marmionii” Strain of Rickettsia honei, Eastern Australia

Nathan B. Unsworth*, John Stenos*

, Stephen R. Graves*, Antony G. Faa†, G. Erika Cox‡, John R. Dyer§, Craig S. Boutlis¶, Amanda M. Lane#, Matthew D. Shaw#, Jennifer Robson**, and Michael D. Nissen††

Author affiliations: *Australian Rickettsial Reference Laboratory, Geelong, Victoria, Australia; †Warwick Hospital (Southern Downs Health Services District), Warwick, Queensland, Australia; ‡Launceston General Hospital, Launceston, Tasmania, Australia; §Fremantle Hospital, Fremantle, Western Australia, Australia; ¶Menzies School of Health Research, Darwin, Northern Territory, Australia; #University of Queensland, Brisbane, Queensland, Australia; **Sullivan Nicolaides Pathology, Brisbane, Queensland, Australia; ††Royal Brisbane Hospital, Brisbane, Queensland, Australia

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Oligonucleotide primers used for PCR amplification and sequencing

Primer	Nucleotide sequence (5′→3′)	Gene	Reference
CS-F	TCG CAA ATG TTC ACG GTA CTT T	gltA	29
CS-R	TCG TGC ATT TCT TTC CAT TGT G	gltA	29
CS-P*	TGC AAT AGC AAG AAC CGT AGG CTG GAT G	gltA	29
MTO-1	GCT CTT GCA ACT CTA TGT T	orf17	12
MTO-2	CAT TGT TCG TCA GGT TGG CG	orf17	12
CS-162-F	GCA AGT ATC GGT GAG GAT GTA ATC	gltA	15
CS-398-SF	5′ATT ATG CTT GCG GCT GTC GG	gltA	15
CS-731-SR	AAG CAA AAG GGT TAG CTC C	gltA	15
RpCS1258	ATT GCA AAA AGT ACA GTG AAC A	gltA	17
rRNA1	AGA GTT TGA TCC TGG CTC AG	rrs	16
rRNA2	AAG GAG GTG ATC CAG CCG CA	rrs	16
rRNA3	CCC TCA ATT CCT TTG AGT TT	rrs	16
rRNA4	CAG CAG CCG CGG TAA TAC	rrs	16
Rr190.70p	ATG GCG AAT ATT TCT CCA AAA	ompA	17
Rr190.602n	AGT GCA GCA TTC GCT CCC CCT	ompA	17
D1f	ATG AGT AAA GAC GGT AAC CT	sca4	18
D928r	AAG CTA TTG CGT CAT CTC CG	sca4	18
D767f	CGA TGG TAG CAT TAA AAG CT	sca4	18
D1390r	CTT GCT TTT CAG CAA TAT CAC	sca4	18
D1219f	CCA AAT CTT CTT AAT ACA GC	sca4	18
D1876r	TAG TTT GTT CTG CCA TAA TC	sca4	18
D1738f	GTA TCT GAA TTA AGC AAT GCG	sca4	18
D2482r	CTA TAA CAG GAT TAA CAG CG	sca4	18
D2338f	GAT GCA GCG AGT GAG GCA GC	sca4	18
D3069r	TCA GCG TTG TGG AGG GGA AG	sca4	18

*5′ end labeled with 6-FAM; 3′ end labeled with BHQ-1.

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Page created: January 04, 2012

Page updated: January 04, 2012

Page reviewed: January 04, 2012

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