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Volume 13, Number 4—April 2007
Dispatch

MRSA Transmission between Cows and Humans

Éva Juhász-Kaszanyitzky*, Szilárd Jánosi*, Pál Somogyi*, Ádám Dán*, Linda vanderGraaf van Bloois†‡, Engeline van Duijkeren‡, and Jaap A. Wagenaar†‡
Author affiliations: *Central Veterinary Institute, Budapest, Hungary; †Animal Sciences Group, Lelystad, the Netherlands; ‡Utrecht University, Utrecht, the Netherlands;

Main Article

Table

Susceptibility of human and animal Staphylococcus aureus strains to phages and the presence of mecA by PCR*

Strain no.†Reference no.Identification of samplesmecAMRSA 100× RTD‡*
1
13535
795 LF
+
616/617/623/626/630
2
16480
588 RF
+
616/617/623/626/630
3
24069/2
490 RF
+
616/617/623/626/630
4
24069/4
723 RH
+
616/617/623/626/630
5
24069/13
1572 LF
+
616/617/623/626/630
6
30195
632 RF
+
616/617/623/626/630
7
23457
1379 LH
+
616/617/623/626/630
29509†
540 RH
+
616/617/623/626/630
8
Human/3
+
616/617/623/626/630
9
24069/9
381 LF
–
10
24069/10
429 LH
–
11
24069/11
519 LH
–
12
24069/15
2551 RH
–
13
Human/4
–
14Human/2–

*Strains isolated from cows with subclinical mastitis, Hungary, January 2002–December 2004.
†See Figure for DNA analysis. Strain no. 29509 was not included in the DNA analysis; thus, no strain no. was assigned.
‡Reaction pattern with 100× routine test dilution (RTD) methicillin-resistant Staphyloccocus aureus phages (7). +, positive in mecA PCR; –,
negative in mecA PCR.

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References
  1. Van Duijkeren E, Box ATA, Heck MEOC, Wannet WJB, Fluit AC. Methicillin-resistant staphylococci isolated from animals.Vet Microbiol. 2004;103:91–7. DOIPubMedGoogle Scholar
  2. Seguin JC, Walker R, Caron JP, Kloos WE, George CG, Hollis RJ, Methicillin-resistant Staphylococcus aureus outbreak in a veterinary teaching hospital: potential human-to-animal transmission.J Clin Microbiol. 1999;37:1459–63.PubMedGoogle Scholar
  3. Van Duijkeren E, Wolfhagen MJHM, Box ATA, Heck MEOC, Wannet WJB, Fluit AC. Human-to-dog transmission of methicillin-resistant Staphylococcus aureus.Emerg Infect Dis. 2004;10:2235–7.PubMedGoogle Scholar
  4. Voss A, Loeffen F, Bakker J, Klaassen C, Wulf M. Methicillin-resistant Staphylococcus aureus in pig farming.Emerg Infect Dis. 2005;11:1965–6.PubMedGoogle Scholar
  5. Jaffe RI, Lane JD, Albury SV, Niemeyer DM. Rapid extraction from and direct identification in clinical samples of methicillin-resistant staphylococci using the PCR.J Clin Microbiol. 2000;38:3407–12.PubMedGoogle Scholar
  6. Richardson JF, Chittasobhon N, Marples RR. Supplementary phages for the investigation of strains of methicillin-resistant Staphylococcus aureus.J Med Microbiol. 1988;25:67–74.PubMedGoogle Scholar
  7. McDougal LK, Steward CD, Killgore GE, Chaitram JM, McAllister SK, Tenover FC. Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States: establishing a national database.J Clin Microbiol. 2003;41:5113–20. DOIPubMedGoogle Scholar
  8. Shopsin B, Gomez M, Montgomery SO, Smith DH, Waddington M, Dodge DE, Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains.J Clin Microbiol. 1999;37:3556–63.PubMedGoogle Scholar
  9. Enright MC, Day NPJ, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus.J Clin Microbiol. 2000;38:1008–15.PubMedGoogle Scholar
  10. Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.J Clin Microbiol. 2005;43:5026–33. DOIPubMedGoogle Scholar
  11. Vandenesch F, Naimi T, Enright MC, Lina G, Nimmo GR, Hefferman H, Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence.Emerg Infect Dis. 2003;9:978–84.PubMedGoogle Scholar
  12. Conceição T, Aires de Sousa M, Füzi M, Tóth Á, Pászti J, Ungvári E, Massive spread of the New York/Japan MRSA clone (ST5-SCCmec II) in a European country. In: Abstracts of the16th European Congress of Clinical Microbiology and Infectious Diseases. Nice, April 1–4, 2006. Basel (Switzerland): European Society of Clinical Microbiology and Infectious Diseases; 2006.
  13. Layer F, Ghebremedhin B, König W, König B. Heterogeneity of methicillin-susceptible Staphylococcus aureus strains at a German university hospital implicates the circulating-strain pool as a potential source of emerging methicillin-resistant S. aureus clones.J Clin Microbiol. 2006;44:2179–85. DOIPubMedGoogle Scholar
  14. Van Leeuwen WB, Melles DC, Alaidan A, Al-Ahdal M, Boelens HAM, Snijders SV, Host- and tissue-specific pathogenic traits of Staphylococcus aureus.J Bacteriol. 2005;187:4584–91. DOIPubMedGoogle Scholar
  15. Lee JH. Methicillin (oxacillin)–resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans.Appl Environ Microbiol. 2003;69:6489–94. DOIPubMedGoogle Scholar

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