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Volume 13, Number 8—August 2007
Letter

Mycobacterium cosmeticum, Ohio and Venezuela

Robert C. Cooksey*Comments to Author , Jacobus H. de Waard†, Mitchell A. Yakrus*, Sean R. Toney*, Omaira Da Mata†, Scott Nowicki‡, Kevin Sohner‡, Elizabeth Koch‡, Cathy A. Petti§, Roger E. Morey*, and Arjun Srinivasan*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †Instituto de Biomedicina, Caracas, Venezuela; ‡Ohio Department of Health, Columbus, Ohio, USA; §ARUP Laboratories, Salt Lake City, Utah, USA;

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Figure

Repetitive element (Rep)–PCR (A) and pulsed-field gel electrophoresis (PFGE) (B) patterns of Mycobacterium cosmeticum isolates from 2 patients in Ohio and 1 patient in Venezuela. Rep-PCR was performed by using BOXA1R primer (3), and PFGE was performed with restriction enzyme AseI. Lanes 1, 2, Ohio isolates OH1 and OH2; lanes 3, 4, control strains ATCC BAA-878T and ATCC BAA-879; lane 5, Venezuelan isolate VZ1. DNA size standards are 100-bp (S1) and 48.5-kb marker (S2).

Figure. Repetitive element (Rep)–PCR (A) and pulsed-field gel electrophoresis (PFGE) (B) patterns of Mycobacterium cosmeticum isolates from 2 patients in Ohio and 1 patient in Venezuela. Rep-PCR was performed by using BOXA1R primer (3), and PFGE was performed with restriction enzyme AseI. Lanes 1, 2, Ohio isolates OH1 and OH2; lanes 3, 4, control strains ATCC BAA-878T and ATCC BAA-879; lane 5, Venezuelan isolate VZ1. DNA size standards are 100-bp (S1) and 48.5-kb marker (S2).

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References
  1. Cooksey  RC, de Waard  JH, Yakrus  MA, Rivera  I, Chopite  M, Toney  SR, Mycobacterium cosmeticum sp. nov., a novel rapidly growing species isolated from a cosmetic infection and from a nail salon. Int J Syst Evol Microbiol. 2004;54:2385–91. DOIPubMedGoogle Scholar
  2. Pfyffer  GE, Brown-Elliott  BA, Wallace  RJ Jr. Mycobacterium: general characteristics, isolation, and staining procedures. In: Murray PR, Baron EJ, Pfaller MA, Jorgensen JH, Yolken RH, editors. Manual of clinical microbiology. Washington: ASM Press; 2003. p. 532–59.
  3. Dombek  PE, Johnson  LK, Zimmerley  ST, Sadowsky  MJ. Use of repetitive DNA sequences and the PCR to differentiate Escherichia coli isolates from human and animal sources. Appl Environ Microbiol. 2000;66:2572–7. DOIPubMedGoogle Scholar
  4. Wagner  D, Young  LS. Nontuberculous mycobacterial infections: a clinical review. Infection. 2004;32:257–70. DOIPubMedGoogle Scholar

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Page updated: June 30, 2010
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