Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 14, Number 11—November 2008
Dispatch

Metagenomic Diagnosis of Bacterial Infections

Shota Nakamura, Norihiro Maeda, Ionut Mihai Miron, Myonsun Yoh, Kaori Izutsu, Chidoh Kataoka, Takeshi Honda, Teruo Yasunaga, Takaaki Nakaya, Jun Kawai, Yoshihide Hayashizaki, Toshihiro Horii, and Tetsuya IidaComments to Author 
Author affiliations: Osaka University, Suita, Japan (S. Nakamura, I.M. Miron, M. Yoh, K. Izutsu, C. Kataoka, T. Honda, T. Yasunaga, T. Nakaya, T. Horii, T. Iida); RIKEN Yokohama Institute, Yokohama, Japan (N. Maeda, J. Kawai, Y. Hayashizaki);

Main Article

Table

Frequency distributions of species in fecal samples taken from patient during illness and after recovery, as determined by BLASTN*

Organism No. (%)
Illness† Recovery‡
Bacteroides vulgatus 5,944 (50.5) 4,743 (56.5)
Homo sapiens 2,955 (25.1) 84 (1.0)
Parabacteroides distasonis 818 (6.9) 1,283 (15.3)
B. thetaiotaomicron 767 (6.5) 1,046 (12.5)
B. fragilis 759 (6.4) 842 (10.0)
Uncultured bacterium 195 (1.7) 227 (2.7)
Campylobacter jejuni 156 (1.3) 0
B. ovatus 48 (0.4) 63 (0.8)
Uncultured Bacteroides spp. 20 (0.2) 19 (0.2)
B. uniformis 14 (0.1) 8 (0.1)

*BLASTN available from http://blast.ncbi.nlm.nih.gov. Expect-value cutoff 10–40.
†Diarrheic fecal sample collected while patient was ill. Total sequences 96,941; total (100%) BLAST matches 11,777.
‡Nondiarrheic fecal sample collected 3 mo after patient had recovered. Total sequences 106,327; total (100%) BLAST matches 8,397.

Main Article

Page created: July 21, 2010
Page updated: July 21, 2010
Page reviewed: July 21, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external