Volume 14, Number 2—February 2008
Experimental Infection and Natural Contact Exposure of Dogs with Avian Influenza Virus (H5N1)
|Dog no.||EID50||Body temperature (dpi)
||Pharyngeal swab (dpi)
|5 (dog contact)||0||38.3||38.4||38.0||38.7||–||–||–||–||21||–||–|
|6 (cat contact)||0||38.4||38.2||38.4||38.5||–||–||–||–||21||–||–|
|7 (cat contact)||0||38.3||38.5||38.6||38.8||–||–||–||–||21||–||–|
|8 (cat contact)||0||38.4||38.5||38.2||38.3||–||–||–||–||21||–||–|
|9 (neg. control)||0||38.4||38.2||38.7||37.8||–||–||–||–||21||–||–|
|10 (neg. control)||0||38.3||38.6||38.8||38.0||–||–||–||–||21||–||–|
*Elevated body temperatures (above 39°C) are underlined. Real time reverse transcription–PCR results are given as – (cycle threshold [ct] values >38) and + (ct values 31–38). Animal serum samples were examined by hemagglutination-inhibition (HI) using highly pathogenic influenza virus (H5N1) strain A/cat/Germany/R606/2006 (9) as antigen. Results are given as reciprocal titers. Competitive nucleoprotein ELISA results are given as – (inhibition <65%) and + (inhibition >65); EID50, 50% egg infectious dose; dpi, days postinfection.
†Serum from dog no. 1 on dpi 10 showed 39.4% inhibition.
‡Serum from dog no. 2 showed 21.6% inhibition.
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