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Volume 14, Number 4—April 2008
Dispatch

Single Nucleotide Polymorphism Typing of Bacillus anthracis from Sverdlovsk Tissue

Richard T. Okinaka*†, Melinda Henrie*, Karen K. Hill*, KristinS. Lowery‡, Matthew Van Ert†, Talima Pearson†, James Schupp†, Leo Kenefic†, Jodi Beaudry†, Steven A. Hofstadler‡, Paul J. Jackson*§, and Paul Keim*†¶Comments to Author 
Author affiliations: *Los Alamos National Laboratory, Los Alamos, New Mexico, USA; †Northern Arizona University, Flagstaff, Arizona, USA; ‡Ibis Biosciences Inc, Carlsbad, California, USA; §Lawrence Livermore National Laboratory, Livermore, California, USA; ¶Translational Genomics Research Institute, Phoenix, Arizona, USA;

Main Article

Table

Canonical single nucleotide polymorphism (canSNP) typing and a new pagA SNP from Sverdlovsk 7.RA93.15.15 (spleen)*
View Actual Table

Isolate Country of origin Lineage/group (10) canSNP,
A.Br.007 canSNP,
A.Br.008 canSNP,
A.Br.009 SNP,
pagA 981
A1055 C.Br.A055 T T A –pXO1
KrugerB B.Br.KrugerB T T A A
CNEVA.9066 B.Br.CNEVA T T A A
Ames A.Br.Ames T T A A
Sterne A.Br.Ames T T A A
Australia94 A.Br.Aust94 T T A A
Vollum A.Br.Vollum C T A A
(Branch point) A.Br.008/009 T G A
Sv7,RA93.15.15 (spleen) A.Br.008/009 T G A T
Sv31.RA93.39.3 T
Sv40.RA93.40.5 T
Sv25.RA93.031 T
Sv1.RA93.42.1 T
Sv33.RA93.20.5 T
Sv21.RA93.38.4 T
Western North America A.Br.WNA T G G A
A0362 Norway A.Br.008/009 T G A T
A0417 Hungary A.Br.008/009 T G A T
A0324 Slovakia A.Br.008/009 T G A T
A0293 Italy A.Br.008/009 T G A A
A0463 Pakistan A.Br.008/009 T G A A
A0149 Turkey A.Br.008/009 T G A A
A0264 Turkey A.Br.008/009 T G A A
A0032 China A.Br.008/009 T G A A
A0033 China A.Br.008/009 T G A A
A0241 Turkey A.Br.008/009 T G A A
A0245 Turkey A.Br.008/009 T G A A
A0463 Pakistan A.Br.008/009 T G A A

*This table illustrates 2 canSNPs that distinguish the subgroup/branch point A.Br.008/009 from the Western North American (WNA) sublineage. The table also depicts the status of the pagA 981 SNP in 7 of 12 Sverdlovsk samples and several other key isolates. Black shading, WNA lineage; gray shading, subgroup A.Br.008/009.

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References
  1. Meselson  M, Guillemin  J, Hugh-Jones  M, Langmuir  A, Popova  I, Shelokov  A, The Sverdlovsk anthrax outbreak of 1979. Science. 1994;266:1202–8. DOIPubMedGoogle Scholar
  2. Abramova  FA, Grinberg  LM, Yampolskaya  OV, Walker  DH. Pathology of inhalational anthrax in 42 cases from the Sverdlovsk outbreak of 1979. Proc Natl Acad Sci U S A. 1993;90:2291–4. DOIPubMedGoogle Scholar
  3. Jackson  PJ, Hugh-Jones  ME, Adair  DM, Green  G, Hill  KK, Kuske  CR, PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims: the presence of multiple Bacillus anthracis strains in different victims. Proc Natl Acad Sci U S A. 1998;95:1224–9. DOIPubMedGoogle Scholar
  4. Jackson  PJ, Walthers  EA, Kalif  AS, Richmond  KL, Adair  DM, Hill  KK, Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates. Appl Environ Microbiol. 1997;63:1400–5.PubMedGoogle Scholar
  5. Price  LB, Hugh-Jones  M, Jackson  PJ, Keim  P. Genetic diversity in the protective antigen gene of Bacillus anthracis. J Bacteriol. 1999;181:2358–62.PubMedGoogle Scholar
  6. Keim  P, Kalif  A, Schupp  J, Hill  K, Travis  SE, Richmond  K, Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers. J Bacteriol. 1997;179:818–24.PubMedGoogle Scholar
  7. Pearson  T, Busch  JD, Ravel  J, Read  TD, Rhoton  SD, U’Ren  JM, Phylogenetic discovery bias in Bacillus anthracis using single-nucleotide polymorphisms from whole-genome sequencing. Proc Natl Acad Sci U S A. 2004;101:13536–41. DOIPubMedGoogle Scholar
  8. Worobey  M. Anthrax and the art of war (against ascertainment bias). Heredity. 2005;94:459–60. DOIPubMedGoogle Scholar
  9. Keim  P, Van Ert  MN, Pearson  T, Vogler  AJ, Huynh  LY, Wagner  DM. Anthrax molecular epidemiology and forensics: using the appropriate marker for different evolutionary scales. Infect Genet Evol. 2004;4:205–13. DOIPubMedGoogle Scholar
  10. Van Ert  MN, Easterday  WR, Huynh  LY, Okinaka  RT, Hugh-Jones  ME, Ravel  J, Global genetic population structure of Bacillus anthracis.. PLoS One. 2007;2:e461. DOIPubMedGoogle Scholar
  11. Van Ert  MN, Hofstadler  SA, Jiang  Y, Busch  JD, Wagner  DM, Drader  JJ, Mass spectrometry provides accurate characterization of two genetic markers in Bacillus anthracis. Biotechniques. 2004;37:642–51.PubMedGoogle Scholar
  12. Hanson  RP. The earliest account of anthrax in man and animals in North America. J Am Vet Med Assoc. 1959;135:463–5.PubMedGoogle Scholar
  13. Sue  D, Marston  CK, Hoffmaster  AR, Wilkins  PP. Genetic diversity in a historical collection of Bacillus anthracis (1954–1988). J Clin Microbiol. 2007;45:1777–82. DOIPubMedGoogle Scholar
  14. Van Ert  MN, Easterday  WR, Simonson  TS, U’Ren  JM, Pearson  T, Kenefic  LJ, Strain-specific single-nucleotide polymorphism assays for the Bacillus anthracis Ames strain. J Clin Microbiol. 2007;45:47–53. DOIPubMedGoogle Scholar
  15. Alibeck  K, Handelman  S. Biohazard: the chilling true story of the largest covert biological weapons program in the world. New York: Dell Publishing; 1999.

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