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Volume 14, Number 4—April 2008
Research

Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA

Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*Comments to Author 
Author affiliations: *Commissariat à l’Energie Atomique, Gif-sur-Yvette, France; †National Veterinary Institute, Oslo, Norway; ‡Institut National de la Recherche Agronomique-Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; §Infectiologie Animale et Santé Publique, Tours, France; ¶Bio-Rad, Marnes-la-Coquette, France; #Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;

Main Article

Figure 5

Analysis of different ovine strains by ELISA typing test and immunoblot. A) ELISA typing test. Three Nor98 isolates (Fiksdal, Stranda, and Seim) were analyzed by using the ELISA typing test. Absorbances obtained in the classic A′typing reagent are close to 0, preventing calculation of the A/A′ratio. Ratios obtained for a Norwegian scrapie isolate (Lavik) and for the 3 internal controls (classic Scr 99–1316, intermediate scrapie PG1259, and experimental ovine bovine spongiform encephalopathy [BSE] 397 BS) are indicated. B) Pattern of migration of different ovine strains. Lanes 1 and 9, untreated negative brain homogenate. Lanes 2–8, proteinase K–treated brain homogenates: French scrapie isolate 99–1316 (lane 2); experimental ovine BSE 397 BS (lanes 3 and 8); Norwegian scrapie isolate (Lavik) (lane 4); Nor98 Stranda and Nor98 Seim scrapie isolates (lanes 5 and 6, respectively); negative brain homogenate (lane 7). MW, molecular weight.

Figure 5. Analysis of different ovine strains by ELISA typing test and immunoblot. A) ELISA typing test. Three Nor98 isolates (Fiksdal, Stranda, and Seim) were analyzed by using the ELISA typing test. Absorbances obtained in the classic A′typing reagent are close to 0, preventing calculation of the A/A′ratio. Ratios obtained for a Norwegian scrapie isolate (Lavik) and for the 3 internal controls (classic Scr 99–1316, intermediate scrapie PG1259, and experimental ovine bovine spongiform encephalopathy [BSE] 397 BS) are indicated. B) Pattern of migration of different ovine strains. Lanes 1 and 9, untreated negative brain homogenate. Lanes 2–8, proteinase K–treated brain homogenates: French scrapie isolate 99–1316 (lane 2); experimental ovine BSE 397 BS (lanes 3 and 8); Norwegian scrapie isolate (Lavik) (lane 4); Nor98 Stranda and Nor98 Seim scrapie isolates (lanes 5 and 6, respectively); negative brain homogenate (lane 7). MW, molecular weight.

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Page updated: July 14, 2010
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