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Volume 14, Number 4—April 2008

Research

Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA

Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*Comments to Author 
Author affiliations: *Commissariat à l’Energie Atomique, Gif-sur-Yvette, France; †National Veterinary Institute, Oslo, Norway; ‡Institut National de la Recherche Agronomique-Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; §Infectiologie Animale et Santé Publique, Tours, France; ¶Bio-Rad, Marnes-la-Coquette, France; #Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;

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Appendix Figure 1

Sensitivity of the ELISA typing test. A) ARQ/ARQ genotype. Fifteen experimental ovine bovine spongiform encephalopathy (BSE) animals from a first passage and 10 from a second passage were tested by using the typing ELISA, in at least 3 independent experiments. For some animals, different regions of the central nervous system were tested (animals SB1, 359, 397, 7704, and 7705). SC, spinal cord. BS, brain stem; FC, frontal cortex; PC, parietal cortex; OC, occipital cortex. B) ARR/ARR genotype. Seven experimental ovine BSE animals from a first passage were tested by using the typing ELISA, in at least 4 independent experiments.

Appendix Figure 1. Sensitivity of the ELISA typing test. A) ARQ/ARQ genotype. Fifteen experimental ovine bovine spongiform encephalopathy (BSE) animals from a first passage and 10 from a second passage were tested by using the typing ELISA, in at least 3 independent experiments. For some animals, different regions of the central nervous system were tested (animals SB1, 359, 397, 7704, and 7705). SC, spinal cord. BS, brain stem; FC, frontal cortex; PC, parietal cortex; OC, occipital cortex. B) ARR/ARR genotype. Seven experimental ovine BSE animals from a first passage were tested by using the typing ELISA, in at least 4 independent experiments.

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