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Volume 14, Number 7—July 2008


Proficiency of Nucleic Acid Tests for Avian Influenza Viruses, Australasia

Sacha Stelzer-Braid*†, Ros Escott‡, Cristina Baleriola*, Peter D. Kirkland§, Peter Robertson*, Michael Catton¶, and William D. Rawlinson*†Comments to Author 
Author affiliations: *Prince of Wales Hospital, Randwick, New South Wales, Australia; †University of New South Wales, Kensington, New South Wales, Australia; ‡Royal College of Pathologists of Australasia, North Ryde, New South Wales, Australia; §Elizabeth Macarthur Agricultural Institute, Camden, New South Wales, Australia; ¶Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia;

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Table 1

Avian influenza quality assurance panel specimen details, Australasia

Specimen Dilution Copy number/μL
Influenza A (H5N1) Indonesian 1:1,000 6.25 × 103
Influenza A (H5N1) Vietnamese 1:1,000 5 × 103
Influenza A (H5N1) Indonesian 1:100,000 6.25 × 101
MDCK-negative control 1:1,000 NA
Influenza A (H5N1) Vietnamese 1:100,000 5 × 101
Influenza A (H3N2) (isolated in Sydney, New South Wales, Australia; close sequence match to A/Canterbury/29/2005) 1:1,000 1.25 × 102
Influenza B (isolated in Sydney; no sequence information available) 1:1,000 2 × 102
Influenza A (H7N4) (A/emu/NSW/97) 1:1,000 1 × 104
Influenza A (H5N1) Indonesian* 1:10,000,000 6.25 ×10–1
Influenza A (H5N1) Vietnamese* 1:10,000,000† 5 × 10–1

*These dilutions were included in panels 2 and 3 only; NA, not applicable.
†For panel 3, the dilution for the influenza (H5N1) Vietnamese strain was changed to 1:1,000,000, with a copy number of 5 × 100 μL.

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