Volume 14, Number 7—July 2008
Dispatch
Proficiency of Nucleic Acid Tests for Avian Influenza Viruses, Australasia
Table 2
Summary of avian influenza quality assurance project, Australasia*
Panel no.† | Date | % Correct results, Indonesian strain‡ | % Correct results, Vietnamese strain‡ | % Testing for influenza A matrix | Most common extraction method (% participants) | Most common amplification method (% participants) |
---|---|---|---|---|---|---|
1 | 2006 Oct 7 | 87.5 | 80 | 46 | QIAGEN QIAamp viral RNA minikit (50) | QIAGEN Artus Influenza/H5 LC RT-PCR kit (20) |
2 | 2006 Nov 9 | 84 | 84 | 73 | QIAGEN QIAamp viral RNA minikit (50) | Invitrogen Superscript III qRT-PCR (31) |
3 | 2006 Jun 11 | 82 | 88 | 75 | QIAGEN QIAamp viral RNA minikit (50) | Invitrogen Superscript III qRT-PCR (40) |
*RT-PCR, reverse transcription–PCR.
†Panels 2 and 3 had 2 very low dilutions of subtype H5N1 that were beyond the limit of detection for most laboratories. Percentage of correct results reported for the Indonesian and Vietnamese strains, with these results included, is 59 and 59 for panel 2 and 56 and 66 for panel 3, respectively.
‡Results reported for influenza H5/(H5N1) testing.