Volume 14, Number 8—August 2008
Research
Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007
Robert S. Lanciotti*
, Olga L. Kosoy*, Janeen J. Laven*, Jason O. Velez*, Amy J. Lambert*, Alison J. Johnson*, Stephanie M. Stanfield*, and Mark R. Duffy*
, Olga L. Kosoy*, Janeen J. Laven*, Jason O. Velez*, Amy J. Lambert*, Alison J. Johnson*, Stephanie M. Stanfield*, and Mark R. Duffy*
Table 3
Description and performance characteristics of Zika virus real-time RT-PCR primer/probe sets*
| Primer | Genome position† | Sequence (5′ → 3′) | Sensitivity, no. copies | Specificity‡ |
|---|---|---|---|---|
| ZIKV 835 | 835–857 | TTGGTCATGATACTGCTGATTGC | ||
| ZIKV 911c | 911–890 | CCTTCCACAAAGTCCCTATTGC | 100 | ZIKV |
| ZIKV 860-FAM | 860–886 | CGGCATACAGCATCAGGTGCATAGGAG | ||
| ZIKV 1086 | 1086–1102 | CCGCTGCCCAACACAAG | ||
| ZIKV 1162c | 1162–1139 | CCACTAACGTTCTTTTGCAGACAT | 25 | ZIKV |
| ZIKV 1107-FAM | 1107–1137 | AGCCTACCTTGACAAGCAGTCAGACACTCAA |
*RT-PCR, reverse transcription–PCR; ZIKV, Zika virus.
†Based on ZIKV MR 766 GenBank accession no. AY632535.
‡ZIKV specificity indicates a positive result with ZIKV only and no reactivity with dengue virus-1 (DENV-1), DENV-2, DENV-3, DENV-4, West Nile virus, St. Louis encephalitis virus, yellow fever virus, Powassan virus, Semliki Forest virus, o’nyong-nyong virus, chikungunya virus, and Spondweni virus.
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