Volume 14, Number 8—August 2008
Research
Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007
Table 4
Results of quantitative real-time RT-PCR of samples from ZIKV-positive patients, Yap State, Micronesia, 2007*
Patient | Days after onset | ZIKV real-time RT-PCR | |||
---|---|---|---|---|---|
Ct-860† | Ct-1107† | Result | Estimated copies/mL‡ | ||
824 | 1 | 34.3 | 34.7 | + | 11,647 |
939 | 2 | 32.0 | 32.4 | + | 67,817 |
947 | 2 | 34.3 | 33.9 | + | 21,495 |
949 | 2 | 35.1 | 35.1 | + | 8,573 |
969 | 1 | 29.4 | 29.3 | + | 728,800 |
037 | 1 | 32.1 | 32.5 | + | 62,816 |
830a | 2 | 30.7 | 30.0 | + | 426,325 |
847a | 5 | 34.8 | 34.7 | + | 11,647 |
950a | 0 | 32.2 | 32.7 | + | 53,894 |
943 | 3 | 37.6 | 35.6 | + | 5,845 |
952 | 1 | 29.3 | 29.5 | + | 625,280 |
958 | 11 | 29.9 | 30.3 | + | 338,797 |
970 | 1 | 35.5 | 34.8 | + | 10,788 |
42 | 0 | 32.9 | 33.6 | + | 27,048 |
941 | 3 | 31.1 | 38.0 | + | 930 |
964 | 0 | 38.3 | 37.6 | + | 1,263 |
063a | 2 | 37.5 | 38.0 | + | 930 |
*RT-PCR, reverse transcription–PCR; ZIKV, Zika virus; Ct, crossing threshold; +, positive.
†Ct values with primer set 835/911c/860-FAM or 1086/1162c/1107-FAM. Values <38.5 are positive.
‡Estimated by testing quantitated dilutions of ZIKV RNA transcripts and standard curve calculation generated by the iCycler instrument (Bio-Rad, Hercules, CA, USA; see Methods).