Volume 14, Number 8—August 2008
Research
Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007
Robert S. Lanciotti*
, Olga L. Kosoy*, Janeen J. Laven*, Jason O. Velez*, Amy J. Lambert*, Alison J. Johnson*, Stephanie M. Stanfield*, and Mark R. Duffy*
, Olga L. Kosoy*, Janeen J. Laven*, Jason O. Velez*, Amy J. Lambert*, Alison J. Johnson*, Stephanie M. Stanfield*, and Mark R. Duffy*
Table 4
Results of quantitative real-time RT-PCR of samples from ZIKV-positive patients, Yap State, Micronesia, 2007*
| Patient | Days after onset | ZIKV real-time RT-PCR | |||
|---|---|---|---|---|---|
| Ct-860† | Ct-1107† | Result | Estimated copies/mL‡ | ||
| 824 | 1 | 34.3 | 34.7 | + | 11,647 |
| 939 | 2 | 32.0 | 32.4 | + | 67,817 |
| 947 | 2 | 34.3 | 33.9 | + | 21,495 |
| 949 | 2 | 35.1 | 35.1 | + | 8,573 |
| 969 | 1 | 29.4 | 29.3 | + | 728,800 |
| 037 | 1 | 32.1 | 32.5 | + | 62,816 |
| 830a | 2 | 30.7 | 30.0 | + | 426,325 |
| 847a | 5 | 34.8 | 34.7 | + | 11,647 |
| 950a | 0 | 32.2 | 32.7 | + | 53,894 |
| 943 | 3 | 37.6 | 35.6 | + | 5,845 |
| 952 | 1 | 29.3 | 29.5 | + | 625,280 |
| 958 | 11 | 29.9 | 30.3 | + | 338,797 |
| 970 | 1 | 35.5 | 34.8 | + | 10,788 |
| 42 | 0 | 32.9 | 33.6 | + | 27,048 |
| 941 | 3 | 31.1 | 38.0 | + | 930 |
| 964 | 0 | 38.3 | 37.6 | + | 1,263 |
| 063a | 2 | 37.5 | 38.0 | + | 930 |
*RT-PCR, reverse transcription–PCR; ZIKV, Zika virus; Ct, crossing threshold; +, positive.
†Ct values with primer set 835/911c/860-FAM or 1086/1162c/1107-FAM. Values <38.5 are positive.
‡Estimated by testing quantitated dilutions of ZIKV RNA transcripts and standard curve calculation generated by the iCycler instrument (Bio-Rad, Hercules, CA, USA; see Methods).
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