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Volume 15, Number 1—January 2009


Isolation of Candidatus Bartonella melophagi from Human Blood1

Ricardo G. Maggi, Michael Y. Kosoy, Melanie Mintzer, and Edward B. BreitschwerdtComments to Author 
Author affiliations: North Carolina State University, Raleigh, North Carolina, USA (R.G. Maggi and E.B. Breitschwerdt); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (M. Kosoy); Generations Family Practice, Cary, North Carolina, USA (M. Mintzer)

Main Article


Sequence similarities for 16S–23S ITS and 3 genes from 2 patient isolates and available GenBank sequences*

Sequence or gene Basepair homology (%) Basepair homology (%)
ITS Bartonella sp. tick† Bartonella melophagi‡
Patient 1 405/408 (99.3) 385/388 (99.2)
Patient 2
405/408 (99.3)
385/388 (99.2)
gltA Bartonella sp. sheep blood§ B. melophagi
Patient 1
131/134 (97.8)
183/187 (97.9)
rpoB B. melophagi#
Patient 1
651/656 (99.2)
16S rRNA Wolbachia melophagi** B. melophagi††
Patient 1 670/671 (99.8) 631/633 (99.7)

*ITS, internal transcribed spacer; gltA, citrate synthase; rpoB, RNA polymerase B; NA, not available.
†Uncultured Bartonella sp. clone BT7498 sequenced from a tick from Peru (GenBank accession no. AF415209).
‡Candidatus B. melophagi strain K-2C isolated from a sheep ked (M. Kosoy, unpub. data).
§Bartonella sp. isolated from commercial sheep blood agar plates (GenBank accession no. EU020109).
¶Candidatus B. melophagi strains K-9B and K-2C isolated from sheep ked (GenBank accession nos. AY724769 and AY724769).
#Candidatus B. melophagi strain K-2C isolated from sheep ked (GenBank accession no. EF605288).
**W. melophagi sequenced from Melophagus ovinus sheep keds (GenBank accession no. X89110).
††Candidatus B. melophagi strain K-2C isolated from a sheep ked (GenBank accession no. AY724770).

Main Article

1Results of this study were presented in part at the 21st Meeting of the American Society for Rickettsiology, Colorado Springs, Colorado, USA, September 8–11, 2007.