Volume 15, Number 11—November 2009
Research
Epidemiology of Hepatitis A Virus Infections, Germany, 2007–2008
Mirko S. Faber, Klaus Stark, Susanne C. Behnke, Eckart Schreier, and Christina Frank
Table 1
Primers used for detection of HAV RNA by nested RT-PCR in clinical specimens from patients in Germany, 2007–2008*
| Primer | Sequence (5′ → 3′) | Orientation, position, and use |
|---|---|---|
| HAV6a | ggA AAT ATT CAg ATT Agg YTg CCT Tgg T | Sense, 2793–2820, reverse transcription and first-round PCR |
| HAV6b | ggg AAC ATT CAg ATY AgA TTg CCW Tgg T | |
| HAV17a | CAA AgC TCT AgT RTC AgC AgT AAT TCC | Antisense, 3300–3326, reverse transcription and first-round PCR |
| HAV17b | CAA AgC CCT AgT RTC AgC AgT CAC TCC | |
| HAV8a | CTT TTg gAT TKg TTT CYA TTC AgA TTg C | Sense, 2882–2908, nested PCR and sequencing |
| HAV7a | gAA AAC TTC ATT ATT TCA TgM TCY TCW gT | Antisense, 3264–3292, nested PCR and sequencing |
*HAV, hepatitis A virus; RT-PCR, reverse transcription–PCR. Positions in the HAV genome are given according to strain HM-175 (GenBank accession no. M14707).
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