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Volume 15, Number 12—December 2009

Dispatch

Diagnostic Assay for Rickettsia japonica

Nozomu Hanaoka, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, and Ichiro TakajoComments to Author 
Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (N. Hanaoka, H. Kawabata, A. Sakata, M. Ogawa, A. Takano, H. Watanabe, T. Kishimoto, I. Kurane, S. Ando); Yamaguchi University School of Medicine, Yamaguchi, Japan (M. Matsutani, Y. Azuma, M. Shirai); Miyazaki Prefectural Institute for Public Health and Environment, Miyazaki, Japan (S. Yamamoto); Ohara General Hospital, Fukushima, Japan (H. Fujita); Gifu University, Gifu, Japan (H. Kawabata, A. Takano, H. Watanabe)

Main Article

Table 1

Reactivity of the real-time PCR for Rickettsia strains used in this study*

Species Strain Isolation source Reference Real-time PCR
Rickettsia asiatica IO-1 Ixodes ovatus ATCC VR-1593 (8) –
IO-2 I. ovatus (8) –
IO-3 I. ovatus (8) –
IO-25 I. ovatus (8) –
IO-38
 I. ovatus
 (8)
 –
R. conorii
 Malish 7
 Human
 ATCC VR 613T
 –
R. heilongjiangensis
 CH8-1
 Haemaphysalis concinna
 (6)
 +
R. helvetica IM-1 Ixodes monospinosus (9) –
IP-1 I. persulcatus (9) –
IP-2 I. persulcatus (10) –
IP-6
 I. persulcatus
 This study
 –
R. honei
 TT-118
 Ixodes sp.
 (11)
 –
R. japonica DT-1 Dermacentor taiwanensis (12) +
YH Human ATCC VR-1363 +
FLA-1 Haemaphysalis flava (9) +
HH-8 H. hystricis This study +
HH-9
 H. hystricis
 This study
 +
R. prowazekii
 breinl
 Human
 (13)
 –
R. rickettsii
 Sheila Smith
 Human
 (14)
 –
R. sibirica
 246
 Human
 ATCC VR-151
 –
R. tamurae AT-1 Amblyomma testudinarium ATCC VR-1594 (12) –
AT-4 A. testudinarium (6) –
AT-13
 A. testudinarium
 (6)
 –
R. typhi
 Wilmington
 Human
 ATCC VR-144
 –
Rickettsia sp. LON LON-2 Haemaphysalis longicornis (6) –
LON-9 H. longicornis (6) –
LON-13 H. longicornis (6) –

*+, positive; –, not detected.

Main Article

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