Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 15, Number 12—December 2009
Dispatch

Diagnostic Assay for Rickettsia japonica

Nozomu Hanaoka, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, and Ichiro TakajoComments to Author 
Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (N. Hanaoka, H. Kawabata, A. Sakata, M. Ogawa, A. Takano, H. Watanabe, T. Kishimoto, I. Kurane, S. Ando); Yamaguchi University School of Medicine, Yamaguchi, Japan (M. Matsutani, Y. Azuma, M. Shirai); Miyazaki Prefectural Institute for Public Health and Environment, Miyazaki, Japan (S. Yamamoto); Ohara General Hospital, Fukushima, Japan (H. Fujita); Gifu University, Gifu, Japan (H. Kawabata, A. Takano, H. Watanabe)

Main Article

Table 1

Reactivity of the real-time PCR for Rickettsia strains used in this study*

Species Strain Isolation source Reference Real-time PCR
Rickettsia asiatica IO-1 Ixodes ovatus ATCC VR-1593 (8)
IO-2 I. ovatus (8)
IO-3 I. ovatus (8)
IO-25 I. ovatus (8)

IO-38
I. ovatus
(8)

R. conorii
Malish 7
Human
ATCC VR 613T

R. heilongjiangensis
CH8-1
Haemaphysalis concinna
(6)
+
R. helvetica IM-1 Ixodes monospinosus (9)
IP-1 I. persulcatus (9)
IP-2 I. persulcatus (10)

IP-6
I. persulcatus
This study

R. honei
TT-118
Ixodes sp.
(11)

R. japonica DT-1 Dermacentor taiwanensis (12) +
YH Human ATCC VR-1363 +
FLA-1 Haemaphysalis flava (9) +
HH-8 H. hystricis This study +

HH-9
H. hystricis
This study
+
R. prowazekii
breinl
Human
(13)

R. rickettsii
Sheila Smith
Human
(14)

R. sibirica
246
Human
ATCC VR-151

R. tamurae AT-1 Amblyomma testudinarium ATCC VR-1594 (12)
AT-4 A. testudinarium (6)

AT-13
A. testudinarium
(6)

R. typhi
Wilmington
Human
ATCC VR-144

Rickettsia sp. LON LON-2 Haemaphysalis longicornis (6)
LON-9 H. longicornis (6)
LON-13 H. longicornis (6)

*+, positive; –, not detected.

Main Article

References
  1. Walker  DH. Rickettsiae and rickettsial infections: the current state of knowledge. Clin Infect Dis. 2007;45:S3944. DOIPubMedGoogle Scholar
  2. Uchida  T, Uchiyama  T, Kumano  K, Walker  DH. Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int J Syst Bacteriol. 1992;42:3035. DOIPubMedGoogle Scholar
  3. Mahara  F, Koga  K, Sawada  S, Taniguchi  T, Shigemi  F, Suto  T, The first report of the rickettsial infections of spotted fever group in Japan: three clinical cases. Kansenshogaku Zasshi. 1985;59:116571.PubMedGoogle Scholar
  4. Fenollar  F, Raoult  D. Molecular diagnosis of bloodstream infections caused by non-cultivable bacteria. Int J Antimicrob Agents. 2007;30:S715. DOIPubMedGoogle Scholar
  5. Fournier  PE, Dumler  SJ, Greub  G, Zhang  J, Wu  Y, Raoult  D. Gene sequence-based criteria for identification of new Rickettsia isolates and description of Rickettsia heilongjiangensis sp. Nov. J Clin Microbiol. 2003;41:545665. DOIPubMedGoogle Scholar
  6. Fujita  H. Cell culture system for isolation of disease agents: 15 years of experience in Ohara Research Laboratory. Annu Rep Ohara Hosp. 2008;48:2142.
  7. Furuya  Y, Katayama  T, Yoshida  Y, Kaiho  I. Specific amplification of Rickettsia japonica DNA from clinical specimens by PCR. J Clin Microbiol. 1995;33:4879.PubMedGoogle Scholar
  8. Fujita  H, Fournier  PE, Takada  N, Saito  T, Raoult  D. Rickettsia asiatica sp. nov., isolated in Japan. Int J Syst Evol Microbiol. 2006;56:23658. DOIPubMedGoogle Scholar
  9. Fournier  PE, Fujita  H, Takada  N, Raoult  D. Genetic identification of rickettsiae isolated from ticks in Japan. J Clin Microbiol. 2002;40:217681. DOIPubMedGoogle Scholar
  10. Ishikura  M, Ando  S, Shinagawa  Y, Matsuura  K, Hasegawa  S, Nakayama  T, Phylogenetic analysis of spotted fever group rickettsiae based on gltA, 17-kDa, and rOmpA genes amplified by nested PCR from ticks in Japan. Microbiol Immunol. 2003;47:82332.PubMedGoogle Scholar
  11. Robertson  RG, Wisseman  CL Jr. Tick-borne rickettsiae of the spotted fever group in West Pakistan. II. Serological classification of isolates from West Pakistan and Thailand: evidence for two new species. Am J Epidemiol. 1973;97:5564.PubMedGoogle Scholar
  12. Fournier  PE, Takada  N, Fujita  H, Raoult  D. Rickettsia tamurae sp. nov., isolated from Amblyomma testudinarium ticks. Int J Syst Evol Microbiol. 2006;56:16735. DOIPubMedGoogle Scholar
  13. Perez Gallardo  F, Fox  JP. Infection of guinea pigs with massive doses of rickettsiae of epidemic and murine typhus. J Immunol. 1948;60:45563.PubMedGoogle Scholar
  14. Dantas-Torres  F. Rocky Mountain spotted fever. Lancet Infect Dis. 2007;7:72432. DOIPubMedGoogle Scholar
  15. Katayama  T, Furuya  Y, Yoshida  Y, Kaiho  I. Spotted fever group rickettsiosis and vectors in Kanagawa Prefecture. Kansenshogaku Zasshi. 1996;70:5618.PubMedGoogle Scholar

Main Article

Page created: December 09, 2010
Page updated: December 09, 2010
Page reviewed: December 09, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external