Volume 15, Number 12—December 2009
Dispatch
Diagnostic Assay for Rickettsia japonica
Nozomu Hanaoka, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, and Ichiro Takajo
Table 2
Application of PCRs for blood clot specimens derived from acute-stage Japanese spotted fever patients*
| Patient no. | Age, y/sex | Days after onset of fever† | Laboratory examinations |
Real-time PCR¶ | |||
|---|---|---|---|---|---|---|---|
| Rickettsia isolation‡ | Serodiagnosis§ | Conventional PCR |
|||||
| Rj5–Rj10 assay | R1–R2 assay | ||||||
| C1 | 83/F | 5 | + | + | – | – | 38.2 ± 0.6 |
| C2 | 35/F | 6 | + | + | – | – | – |
| C3 | 70/M | 6 | + | + | – | – | 37.6 ± 1.3 |
| C4 | 71/M | 3 | + | + | – | – | – |
| C5 | 66/F | 6 | + | + | – | – | 38.4 ± 1.0 |
| C6 | 49/M | 3 | NT | + | – | – | – |
| C7 | 88/F | 5 | NT | + | – | – | 40.7 ± 0.4 |
| C8 | 49/M | 7 | NT | + | – | – | 31.4 ± 1.1 |
| C9 | 65/F | 3 | NT | + | – | – | 36.0 ± 0.5 |
| C10 | 78/M | 4 | NT | + | – | – | 39.0 ± 0.8 |
| C11 | 72/F | 4 | NT | + | – | – | – |
| C12 | 68/F | 5 | NT | + | – | – | – |
| C13 | 27/M | 5 | NT | + | – | – | – |
| C14 | 45/M | 7 | NT | + | – | – | – |
| C15 | 76/F | 5 | NT | + | – | – | 39.0 ± 0.5 |
| C16 | 79/M | 3 | NT | + | – | – | 39.9 ± 1.0 |
| C17 | 69/M | 2 | NT | + | – | – | – |
| C18 | 64/F | 3 | NT | + | – | – | – |
*+, positive; NT, not tested; –, not detected.
†Blood clot was prepared from whole blood at indicated days after onset of fever.
‡Isolation from whole blood specimens.
§Seroconversion was identified by the indirect immunofluorescent test with paired serum specimens.
¶Cycle threshold values are given as means ± standard errors of the means for 3 independent assays.
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