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Volume 15, Number 12—December 2009
Dispatch

CTX-M β-Lactamase Production and Virulence of Escherichia coli K1

Damien Dubois, Nemani V. Prasadarao, Rahul Mittal, Laurent Bret, Marie Roujou-Gris, and Richard BonnetComments to Author 
Author affiliations: University of Auvergne, Clermont-Ferrand, France (D. Dubois, R. Bonnet); Teaching Hospital, Clermont-Ferrand (D. Dubois, R. Bonnet); Childrens Hospital, Los Angeles, California, USA (N.V. Prasadarao, R. Mittal); University of Southern California, Los Angeles (N.V. Prasadarao); La Source Hospital, Orléans, France (L. Bret, M. Roujou-Gris)

Main Article

Table 1

Virulence factors of Orl-1 Escherichia coli K1 strain, France

Integrative elements* (1) Virulence genes Present
PAI III536–like iroN Yes

sfa/foc
Yes
GimA-like
ibeA (gimA4), ptnC (gimA1)
Yes
PAI IIJ96–like hra Yes

hlyC, cnf1
No
PAI ICFT073–like hlyC No

aer (iucC)
Yes
HPI-like
fyuA, irp-2
Yes
GimB-like
gimB
Yes
pks island†
clbA, clbK-J, clbP, clbQ
Yes
Others chuA Yes
ompA Yes
hek Yes
iss Yes
malX Yes
cdtB-I to -V No

*PAI, pathogenicity island; HPI, high-pathogenicity island.
†Positive cytopathogen effect with transient infection of HeLa cells.

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References
  1. Bonacorsi  S, Clermont  O, Houdouin  V, Cordevant  C, Brahimi  N, Marecat  A, Molecular analysis and experimental virulence of French and North American Escherichia coli neonatal meningitis isolates: identification of a new virulent clone. J Infect Dis. 2003;187:1895906. DOIPubMed
  2. Pitout  JD, Laupland  KB. Extended-spectrum beta–lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis. 2008;8:15966. DOIPubMed
  3. Lavigne  JP, Blanc-Potard  AB, Bourg  G, Moreau  J, Chanal  C, Bouziges  N, Virulence genotype and nematode-killing properties of extra-intestinal Escherichia coli producing CTX-M beta-lactamases. Clin Microbiol Infect. 2006;12:1199206. DOIPubMed
  4. Pitout  JD, Laupland  KB, Church  DL, Menard  ML, Johnson  JR. Virulence factors of Escherichia coli isolates that produce CTX-M-type extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 2005;49:466770. DOIPubMed
  5. Branger  C, Zamfir  O, Geoffroy  S, Laurans  G, Arlet  G, Thien  HV, Genetic background of Escherichia coli and extended-spectrum beta-lactamase type. Emerg Infect Dis. 2005;11:5461.PubMed
  6. Andersson  DI. The biological cost of mutational antibiotic resistance: any practical conclusions? Curr Opin Microbiol. 2006;9:4615. DOIPubMed
  7. Houdouin  V, Bonacorsi  S, Bidet  P, Bingen  E. Antimicrobial susceptibility of 136 Escherichia coli isolates from cases of neonatal meningitis and relationship with virulence. Clin Microbiol Infect. 2007;13:120710. DOIPubMed
  8. Galiza  EP, Heath  PT. Improving the outcome of neonatal meningitis. Curr Opin Infect Dis. 2009;22:22934. DOIPubMed
  9. Jonas  D, Spitzmuller  B, Weist  K, Ruden  H, Daschner  FD. Comparison of PCR-based methods for typing Escherichia coli. Clin Microbiol Infect. 2003;9:82331. DOIPubMed
  10. Prasadarao  NV, Wass  CA, Weiser  JN, Stins  MF, Huang  SH, Kim  KS. Outer membrane protein A of Escherichia coli contributes to invasion of brain microvascular endothelial cells. Infect Immun. 1996;64:14653.PubMed
  11. Peigne  C, Bidet  P, Mahjoub-Messai  F, Plainvert  C, Barbe  V, Medigue  C, The plasmid of neonatal meningitis Escherichia coli strain S88 (O45:K1:H7) is closely related to avian pathogenic E. coli plasmids and is associated with high level bacteremia in a neonatal rat meningitis model. Infect Immun. 2009;77:227284. DOIPubMed
  12. Bizzarro  MJ, Dembry  LM, Baltimore  RS, Gallagher  PG. Changing patterns in neonatal Escherichia coli sepsis and ampicillin resistance in the era of intrapartum antibiotic prophylaxis. Pediatrics. 2008;121:68996. DOIPubMed
  13. Boyer-Mariotte  S, Duboc  P, Bonacorsi  S, Lemeland  JF, Bingen  E, Pinquier  D. CTX-M-15–producing Escherichia coli in fatal neonatal meningitis: failure of empirical chemotherapy. J Antimicrob Chemother. 2008;62:14724. DOIPubMed
  14. Ramdani-Bouguessa  N, Mendonca  N, Leitao  J, Ferreira  E, Tazir  M, Canica  M. CTX-M-3 and CTX-M-15 extended-spectrum beta-lactamases in isolates of Escherichia coli from a hospital in Algiers, Algeria. J Clin Microbiol. 2006;44:45846. DOIPubMed
  15. Sehgal  R, Gaind  R, Chellani  H, Agarwal  P. Extended-spectrum beta lactamase–producing gram-negative bacteria: clinical profile and outcome in a neonatal intensive care unit. Ann Trop Paediatr. 2007;27:4554. DOIPubMed

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