Figure 2. Phylogenetic analysis of the PrM-E (A) and nonstructural protein 5 (B) gene sequences of Nanjianyin virus isolated from Yunnan Province, China. Phylogenetic analyses were performed by the neighbor-joining method with MEGA version 3.1 software (www.megasoftware.net). Bootstrap probabilities of each node were calculated with 500 replicates. Scale bars indicate number of nucleotide substitutions per site. Abbreviations and GenBank accession numbers are as follows: tick-borne encephalitis virus (TBEV), TBEV (Far), strain MDJ-01(AY217093); TBEV (Far), strain Senzhang (AY182009); TBEV (Far), strain Sofjin-HO (AB062064); TBEV (Sib), strain Vasilchenko (AF069066); louping iII virus (LIV), strain 369-T2 (Y07863); TBEV (EU), strain Neudoerfl (U27495); Omsk hemorrhagic fever virus (OHFV), strain Bogoluvovska (AY193805); OHFV, strain Kubrin (AY438626); Langat virus (LANV), strain TP21 (AF253419); Alkhurma hemorrhagic fever virus (AHFV), strain 1176 ( AF331718); Kyasanur Forest disease virus (KFDV), strain unknown (PrM-E, X74111); KFDV, strain unknown (EU480689); KFDV, strain Itp6905 (AY323490); KFDV, strain W371 (NS5, AF013385); KFDV, strain Nanjianyin (PrM-E, EU918175; NS5, EU918174); Powassan virus (POWV), strain LB ( L06436); yellow fever virus (YFV), strain Trinidad 79 (AF094612); Japanese encephalitis virus (JEV), strain SA14 (D90194); West Nile virus (WNV), strain B956 (AY532665).