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Volume 15, Number 4—April 2009
Dispatch

Isolation of Genotype V St. Louis Encephalitis Virus in Florida

Christy L. OttendorferComments to Author , Jason H. Ambrose, Gregory S. White, Thomas R. Unnasch, and Lillian M. Stark
Author affiliations: University of South Florida, Tampa, Florida, USA (C.L. Ottendorfer, J.H. Ambrose, G.S. White, T.R. Unnasch, L.M. Stark); Florida Department of Health, Tampa (C.L. Ottendorfer, J.H. Ambrose, L.M. Stark); University of Alabama, Birmingham, Alabama, USA (G.S. White)

Main Article

Table

SLEV strains sequenced for phylogenetic analysis*

Strain Designation Location Year Host Passage GenBank
accession no.
FL52-Miami FL52 Miami, FL 1952 Human SM1, Vero 1 EU906866
TBH-28 TBH-28 Tampa Bay, FL 1962 Human SM11, Vero 2 EU906867
F72-M022 FL72 Walnut Hill, FL 1972 Opossum SM3, Vero 1 EU906868
86-100309 FL85a Indian River, FL† 1985 Culex nigripalpus mosquitoes SM1, Vero 1 EU906869
86-100802 FL85b Indian River, FL 1985 C. nigripalpus mosquitoes SM2, Vero 1 EU906870
1A-059 FL89 Indian River, FL 1989 Northern cardinal SM2, Vero 1 EU906871
3-594 FL90a Indian River, FL 1990 Common grackle SM1, Vero 1 EU906872
3A-038 FL90b Indian River, FL 1990 Mourning dove SM1, Vero 1 EU906873
3-582 FL90c Indian River, FL 1990 Common grackle SM1, Vero 1 EU906874
CXN GR8 FL90d Indian River, FL 1990 C. nigripalpus mosquitoes SM2, Vero 1 EU906875
FL06-S569 FLS569 Sarasota, FL† 2006 Chicken Vero 1 EU906876
FL06-S650 FLS650 Sarasota, FL 2006 Chicken Vero 1 EU906877
TRVL21647 TR58 Trinidad 1958 C. coronator mosquitoes SM3, Vero 1 EU906878
TRVL43174 TR62 Trinidad 1962 C. nigripalpus mosquitoes SM4, Vero 1 EU906879
BeAn70092 BR64 Belem, Brazil 1964 Kingfisher ?, SM1, Vero1 EU906880
BeAn156204 BR69 Belem, Brazil 1969 Chicken SM2, Vero 1 EU906881

*SLEV, St. Louis encephalitis virus; SM, suckling mouse. Twelve strains collected over 5 decades in Florida were sequenced for phylogenetic analysis. Four South American strains of SLEV were acquired by the Bureau of Laboratories–Tampa before 1972 and sequenced as representative of genotype V SLEV. One control strain (TBH-28) was analyzed and used as a positive control in reverse transcription–PCR and sequencing assays. TBH-28 represents a Florida isolate of SLEV made during the 1960s, but the envelope sequence was previously published (GenBank accession no. AF205469).
†Indian River and Sarasota counties.

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Page created: December 10, 2010
Page updated: December 10, 2010
Page reviewed: December 10, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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