Volume 15, Number 6—June 2009
Dispatch
Murine Typhus in Child, Yucatan, Mexico
Table
Methods used to diagnose human Rickettsia typhi infection, Yucatan, Mexico, 2007*
Sample | PCR, % identity |
IFA, titer† |
|||
---|---|---|---|---|---|
Citrate synthase (gltA) (382 bp)‡ | 17-kDa gene (434 bp)§ | IgM | IgG | ||
Rickettsia typhi Wilmington strain | 100 (AE017197.1) | 100 (M28481.1), 99 (AE017197.1) | 256 | 128 | |
R. felis URRWXCal2 | NR | 89 (CP000053.1) | ND | ||
R. rickettsii Sheila Smith | 92 (CP000848.1) | 89 (CP000848.1) | Neg | 128 | |
R. akari Hartford strain | 91 (CP000847.1) | 96 (CP000847.1) | Neg | 64 |
*IFA, indirect immunofluorescence assay; Ig, immunoglobulin; NR, not represented in the first 100 sequences with a significant alignment using BLAST (9); ND, not determined; Neg, negative.
†Titers refer to the reciprocal of the dilution.
‡Primers: RpCS.1258 5′-attgcaaaaa gtaccgtaaaca-3′ and RpCS.877 5′-gccccgccgtgggcaggccccc-3′.
§Primers: 17-kDa 5′-gctcttgcaacttctatgtt-3′ and 5′-cattgttcgtcaggttggcg-3′.
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