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Volume 15, Number 9—September 2009
Dispatch

Highly Pathogenic Avian Influenza Virus A (H7N3) in Domestic Poultry, Saskatchewan, Canada, 2007

Yohannes Berhane, Tamiko Hisanaga, Helen Kehler, James Neufeld, Lisa Manning, Connie Argue, Katherine Handel, Kathleen Hooper-McGrevy, Marilyn Jonas, John Robinson, Robert G. Webster, and John PasickComments to Author 
Author affiliations: Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada (Y. Berhane, T. Hisanaga, H. Kehler, J. Neufeld, L. Manning, C. Argue, K. Handel, K. Hooper-McGrevy, J. Pasick); Prairie Diagnostic Services, Saskatoon, Saskatchewan, Canada (M. Jonas); British Columbia Ministry of Agriculture and Lands, Abbotsford, British Columbia, Canada (J. Robinson); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (R.G. Webster)

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Figure 2

Phyogenetic analysis of avian influenza virus H7 (A) and N3 (B) genes. Trees were generated with MEGA software (8) by using the neighbor-joining method (9). Evolutionary distances were computed by using the method of Nei and Gojobori (10). Percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. Scale bars indicate substitutions per site.

Figure 2. Phyogenetic analysis of avian influenza virus H7 (A) and N3 (B) genes. Trees were generated with MEGA software (8) by using the neighbor-joining method (9). Evolutionary distances were computed by using the method of Nei and Gojobori (10). Percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. Scale bars indicate substitutions per site.

Main Article

References
  1. Webster  RG, Bean  WJ, Gorman  OT, Chambers  TM, Kawaoka  Y. Evoution and ecology of influenza A viruses. Microbiol Rev. 1992;56:152–79.PubMedGoogle Scholar
  2. Spackman  E, Senne  DA, Myers  TJ, Bulaga  LL, Garber  LP, Perdue  ML, Development of a real-time reverse transcriptase PCR assay for type A influenza virus and avian H5 and H7 hemagglutinin subtypes. J Clin Microbiol. 2002;40:3256–60. DOIPubMedGoogle Scholar
  3. Wise  MG, Suarez  DL, Seal  BS, Pedersen  JC, Senne  DA, King  DJ, Development of a real-time reverse-transcription PCR for detection of Newcastle disease virus RNA in clinical samples. J Clin Microbiol. 2004;42:329–38. DOIPubMedGoogle Scholar
  4. World Organisation for Animal Health. Highly pathogenic avian influenza. In: Manual of diagnostic tests and vaccines for terrestrial animals. Paris: The Organisation; 2004. p. 258–69.
  5. Hoffmann  E, Stech  J, Guan  Y, Webster  RG, Perez  DR. Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol. 2001;146:2275–89. DOIPubMedGoogle Scholar
  6. Xing  Z, Cardona  C, Dao  P, Crossley  B, Hietala  S, Boyce  W. Inability of real-time reverse transcriptase PCR assay to detect subtype H7 avian influenza viruses isolated from wild birds. J Clin Microbiol. 2008;46:1844–6. DOIPubMedGoogle Scholar
  7. Zhou  EM, Chan  M, Heckert  RA, Riva  J, Cantin  MF. Evaluation of a competitive ELISA for detection of antibodies against avian influenza virus nucleoprotein. Avian Dis. 1998;42:517–22. DOIPubMedGoogle Scholar
  8. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596–9. DOIPubMedGoogle Scholar
  9. Saitou  N, Nei  M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:406–25.PubMedGoogle Scholar
  10. Nei  M, Gojobori  T. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol. 1986;3:418–26.PubMedGoogle Scholar
  11. Krauss  S, Obert  CA, Franks  J, Walker  D, Jones  K, Seiler  P, Influenza in migratory birds and evidence of limited intercontinental virus exchange. PLoS Pathog. 2007;3:e167. DOIPubMedGoogle Scholar
  12. Parmley  EJ, Bastien  N, Booth  T, Bowes  V, Buck  P, Breault  A, Wild bird influenza survey, Canada, 2005. Emerg Infect Dis. 2008;14:84–7. DOIPubMedGoogle Scholar
  13. Pasick  J, Handel  K, Robinson  J, Copps  J, Ridd  D, Hills  K, Intersegmental recombination between the haemagglutinin and matrix genes was responsible for the emergence of a highly pathogenic H7N3 avian influenza virus in British Columbia. J Gen Virol. 2005;86:727–31. DOIPubMedGoogle Scholar

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