Volume 15, Number 9—September 2009
Dispatch
Merkel Cell Polyomavirus DNA in Persons without Merkel Cell Carcinoma
Ulrike Wieland
, Cornelia Mauch, Alexander Kreuter, Thomas Krieg, and Herbert Pfister
, Cornelia Mauch, Alexander Kreuter, Thomas Krieg, and Herbert Pfister
Table 2
MCPyV DNA and human papillomavirus DNA in samples of HIV-positive men without Merkel cell carcinoma*
| Diagnosis | No. samples | No. (%) MCPyV-positive by nPCR | No. (%) MCPyV-positive by sPCR | No. (%) HPV-positive | No. (%) HR-HPV–positive† |
|---|---|---|---|---|---|
| Normal‡ anogenital/oral swabs | 49 | 18 (37) | 7 (14) | 32 (65) | 31 (63) |
| CD4 <200/μL | 18 | 6 (33) | 3 (17) | 13 (72) | 13 (72) |
| CD4 >200/μL | 31 | 12 (39) | 4 (13) | 19 (61) | 18 (58) |
| Anal | 20 | 6 (30) | 3 (15) | 20 (100) | 20 (100) |
| Penile | 6 | 3 (50) | 3 (50) | 4 (67) | 3 (50) |
| Oral |
23 |
9 (39) |
1 (4) |
8 (35) |
8 (35) |
| Benign papilloma/acanthoma | 21 | 6 (29) | 2 (10) | 20 (95) | 10 (48) |
| Anal | 12 | 4 (33) | 2 (17) | 12 (100) | 5 (42) |
| Penile | 6 | 2 (33) | 0 (0) | 6 (100) | 2 (33) |
| Oral |
3 |
0 (0) |
0 (0) |
2 (67) |
3 (100) |
| Dysplasia/carcinoma in situ | 43 | 12 (28) | 1 (2) | 41 (95) | 38 (88) |
| Anal intraepithelial neoplasia | 27 | 7 (26) | 1 (4) | 26 (96) | 26 (96) |
| Penile intraepithelial neoplasia | 14 | 4 (29) | 0 (0) | 13 (93) | 12 (86) |
| Oral carcinoma in situ |
2 |
1 (50) |
0 (0) |
2 (100) |
0 (0) |
| Anal cancer |
7 |
1 (14) |
0 (0) |
7 (100) |
7 (100) |
| All anal, penile, or oral samples | 120 | 37 (31) | 10 (8) | 100 (83) | 86 (72) |
| CD4 <200/μL | 45 | 13 (29) | 5 (11) | 38 (84) | 36 (80) |
| CD4 >200/μL |
75 |
24 (32) |
5 (7) |
62 (83) |
50 (67) |
| Plucked eyebrow hairs |
14 |
7 (50) |
5 (36) |
14 (100) |
0 (0) |
| Cerebrospinal fluid | 7 | 0 (0) | 0 (0) | ND | ND |
*MCPyV, Merkel cell polyomavirus; nPCR, nested PCR; sPCR, single-round PCR; HPV, human papillomavirus; HR, high-risk alpha-HPV. All samples were tested for beta-HPV. Eyebrow hairs were additionally tested for alpha-HPV, and all were positive. Alpha- and beta-HPV DNA were determined by PCRs as previously described (8).
†HR types comprise HPV16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82. For details of the patient-collective see (8).
‡Normal swabs were those negative for intraepithelial lesion or malignancy.
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