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Volume 16, Number 2—February 2010
Dispatch

Bordetella pertussis Clones Identified by Multilocus Variable-Number Tandem-Repeat Analysis

Jacob Kurniawan, Ram P. Maharjan, Wai-Fong Chan, Peter R. Reeves, Vitali Sintchenko, Gwendolyn L. Gilbert, Frits R. Mooi, and Ruiting LanComments to Author 
Author affiliations: University of New South Wales, Sydney, New South Wales, Australia (J. Kurniawan, R.P. Maharjan, W.-F. Chan, R. Lan); University of Sydney, Sydney (P.R. Reeves, V. Sintchenko, G.L. Gilbert); Westmead Hospital, Westmead, New South Wales, Australia (V. Sintchenko, G.L. Gilbert); National Institute for Public Health and the Environment, Bilthoven, the Netherlands (F.R. Mooi).

Main Article

Table 1

Primers used in study of Bordetella pertussis clones Identified by multilocus variable-number tandem-repeat analysis

Primer name Sequence, 5′ → 3′ Genome coordinates* Mix Concentration,† μM Reference
BP-VNTR1-DF VIC-CCTGGCGGCGGGAGACGTGGTGGTG 2194507 1 0.13 (10)
BP-VNTR1-DR AAAATTGCGGCATGTGGGCTGACTCTGA 2194862 1 (10)
BP-VNTR2-BF VIC-CGCGCCGCCTACGACCGCTATGG 2647550 2 0.08 (10)
BP-VNTR2-BR CCCGCGCCGAAGATCTCGCCAAAGATAT 2647412 2 (10)
BP-VNTR3-BF FAM-GCCTCGGCGAAATTGCTGAAC 2591464 2 0.23 (10)
BP-VNTR3-BR GCGGGCGAGGAAACGCCCGAGACC 2591350 2 (10)
BP-VNTR4-CF NED-CGTGCCCTGCGCCTGGACCTG 185211 2 0.08 (10)
BP-VNTR4-BR GCCGCTGCTCGACGCCAGGGACAA 185000 2 (10)
BP-VNTR5-BF PET-GAAGCCGGCCCACCCGAGCTCCAGGCTCTT 1005290 1 0.06 (10)
BP-VNTR5-BR TGCCGGGTTTCGGCATCTCGATGGGATACG 1005177 1 (10)
BP-VNTR6-EF FAM-CCAACGGCGGTCTGCTGGGTGGTC 2099525 1 0.06 (10)
BP-VNTR6-FR CGCCGCCCGCTGCGCCGCTACC 2099315 1 (10)
VNTR7F2 PET-ATCAGGAAACCCACCACCACGCCGG 124402 2 0.08 This study
VNTR7R2 GTCACCAGCCCGCAGTACTGGCG 124585 2 This study
VNTR8F2 NED-TGGGTGTCTCCGTGATAGTGAGCACTTACAC 444776 1 0.19 This study
VNTR8R2 CTGGCGCAAAAACAGTAAGCCCGCACG 444981 1 This study

*Based on genome sequence position of the Tohama I strain; VNTR, variable-number tandem-repeat.
†Concentrations listed are for forward and reverse primers separately.

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References
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Page updated: December 10, 2010
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