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Volume 16, Number 2—February 2010
Dispatch

Bordetella pertussis Clones Identified by Multilocus Variable-Number Tandem-Repeat Analysis

Jacob Kurniawan, Ram P. Maharjan, Wai-Fong Chan, Peter R. Reeves, Vitali Sintchenko, Gwendolyn L. Gilbert, Frits R. Mooi, and Ruiting LanComments to Author 
Author affiliations: University of New South Wales, Sydney, New South Wales, Australia (J. Kurniawan, R.P. Maharjan, W.-F. Chan, R. Lan); University of Sydney, Sydney (P.R. Reeves, V. Sintchenko, G.L. Gilbert); Westmead Hospital, Westmead, New South Wales, Australia (V. Sintchenko, G.L. Gilbert); National Institute for Public Health and the Environment, Bilthoven, the Netherlands (F.R. Mooi).

Main Article

Table 2

Diversity of variable-number tandem-repeat analysis loci Bordetella pertussis isolates*

Locus No. repeats Global (this study)
Australia (this study)
The Netherlands (10)
No. alleles D No. alleles D No. alleles D
VNTR1 2–12 6 0.58 4 0.63 7 0.26
VNTR2 2–5 4 0.02 2 0.01 3 N/A
VNTR3a 2–8 4 0.40 4 0.43 10 0.18
VNTR3b 0–10 5 0.21 5 0.21 4 0.15
VNTR4 2–9 7 0.34 5 0.24 8 0.21
VNTR5 3–9 6 0.20 4 0.19 7 0.18
VNTR6 2–11 8 0.72 5 0.70 8 0.60
VNTR7 3–4 2 0.01 1 0.00 NA NA
VNTR8 2–4 3 0.16 2 0.03 NA NA

*D, Simpson index of diversity; VNTR, variable-number tandem-repeat; NA, not applicable.

Main Article

References
  1. Tan  T, Trindade  E, Skowronski  D. Epidemiology of pertussis. Pediatr Infect Dis J. 2005;24:S108. DOIPubMed
  2. Guris  D, Strebel  PM, Bardenheier  B, Brennan  M, Tachdjian  R, Finch  E, Changing epidemiology of pertussis in the United States: increasing reported incidence among adolescents and adults, 1990–1996. Clin Infect Dis. 1999;28:12307. DOIPubMed
  3. Celentano  LPMDM, Massari  MD, Paramatti  DD, Salmaso  SD, Tozzi  AE. EUVAC-NET Group. Resurgence of pertussis in Europe. Pediatr Infect Dis J. 2005;24:7615. DOIPubMed
  4. Poynten  M, McIntyre  PB, Mooi  FR, Heuvelman  KJ, Gilbert  GL. Temporal trends in circulating Bordetella pertussis strains in Australia. Epidemiol Infect. 2004;132:18593. DOIPubMed
  5. Bamberger  ES, Srugo  I. What is new in pertussis? Eur J Pediatr. 2008;167:1339. DOIPubMed
  6. Mooi  FR, van Loo  IH, King  AJ. Adaptation of Bordetella pertussis to vaccination: a cause for its reemergence? Emerg Infect Dis. 2001;7:5268. DOIPubMed
  7. Mooi  FR, He  Q, Guiso  N. Phylogeny, evolution, and epidemiology of Bordetellae. In: Locht C, editor. Bordetella molecular microbiology. Wymondham (UK): Horizon Scientific Press; 2007. p. 17–45.
  8. Kodama  A, Kamachi  K, Horiuchi  Y, Konda  T, Arakawa  Y. Antigenic divergence suggested by correlation between antigenic variation and pulsed-field gel electrophoresis profiles of Bordetella pertussis isolates in Japan. J Clin Microbiol. 2004;42:54537. DOIPubMed
  9. Byrne  S, Slack  AT. Analysis of Bordetella pertussis pertactin and pertussis toxin types from Queensland, Australia, 1999–2003. BMC Infect Dis. 2006;6:53. DOIPubMed
  10. Schouls  LM, van der Heide  HG, Vauterin  L, Vauterin  P, Mooi  FR. Multiple-locus variable-number tandem repeat analysis of Dutch Bordetella pertussis strains reveals rapid genetic changes with clonal expansion during the late 1990s. J Bacteriol. 2004;186:5496505. DOIPubMed
  11. Litt  DJ, Neal  SE, Fry  NK. Changes in genetic diversity of the Bordetella pertussis population in the United Kingdom between 1920 and 2006 reflect vaccination coverage and emergence of a single dominant clonal type. J Clin Microbiol. 2009;47:6808. DOIPubMed
  12. Chan  WF, Maharjan  RP, Reeves  PR, Sintchenko  V, Gilbert  GL, Lan  R. Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and high resolution melt analysis. J Microbiol Methods. 2009;77:3269. DOIPubMed
  13. van Amersfoorth  SC, Schouls  LM, van der Heide  HG, Advani  A, Hallander  HO, Bondeson  K, Analysis of Bordetella pertussis populations in European countries with different vaccination policies. J Clin Microbiol. 2005;43:283743. DOIPubMed

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