Volume 16, Number 5—May 2010
Dispatch
La Crosse Virus in Aedes albopictus Mosquitoes, Texas, USA, 2009
Table
Targeted genomic regions | Name | Primer sequence (5′ → 3′) | Approximate amplicon size, bp |
---|---|---|---|
S segment nucleocapsid ORF | Cal S forward | GCAAATGGATTTGATCCTGATGCAG | 210 |
Cal S reverse |
TTGTTCCTGTTTGCTGGAAAATGAT |
||
M segment 5′ terminus/glycoprotein ORF | Ortho M 5′ terminus | AGTAGTGTACTACC | 410 |
Ortho M ORF reverse |
TTRAARCADGCATGGAA |
||
L segment 5′ terminus/polymerase ORF | Ortho L 5′ terminus | AGTAGTGTACTCCTA | 550 |
Ortho L ORF reverse | AATTCYTCATCATCA |
*Oligonucleotide primers designed against conserved regions of the orthobunyavirus genome. S segment primers appear in a previous publication (9). All primers were applied in singleplex reactions using methods described previously (9) with altered primer annealing conditions of 50oC for 1 min. S, small; M, medium; L, large; ORF, open reading frame.
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