Volume 16, Number 6—June 2010
Dispatch
Vaccinia Virus Infection in Monkeys, Brazilian Amazon
Appendix Figure
![Amino acid sequences of vaccinia virus (VACV) samples and comparison with homologous genes sequences from several orthopoxviruses, Brazil. A) Alignment of vaccinia growth factor gene sequences from 6 monkey serum samples showing 100% identity (horizontal box). VACV-TO_CA, sequence from Cebus apella; VACV-TO_AC, sequence from Allouata caraya; HPXV, horsepoxvirus; CPXV, cowpoxvirus; MPXV, monkeypoxvirus; VARV, variola virus; ECMV, ectromelia virus. B) Alignment of orthopoxvirus hemagglutinin gene amino acid sequences showing the deletion signature region (vertical box) in VACV-TO isolates and several VACV strains isolated during bovine vaccinia outbreaks. Arrow indicates polymorphism site in the hemagglutinin amino acid sequences between VACV-TO_CA and VACV-TO_AC. Alignments were made by using ClustalW (www.ncbi.nlm.nih.gov/pmc/articles/PMC308517) and MEGA version 3.1 software (www.megasoftware.net). HSPV, horsepoxvirus.](/eid/images/09-1187-TECHAPP1.jpg)
Appendix Figure. Amino acid sequences of vaccinia virus (VACV) samples and comparison with homologous genes sequences from several orthopoxviruses, Brazil. A) Alignment of vaccinia growth factor gene sequences from 6 monkey serum samples showing 100% identity (horizontal box). VACV-TO_CA, sequence from Cebus apella; VACV-TO_AC, sequence from Allouata caraya; HPXV, horsepoxvirus; CPXV, cowpoxvirus; MPXV, monkeypoxvirus; VARV, variola virus; ECMV, ectromelia virus. B) Alignment of orthopoxvirus hemagglutinin gene amino acid sequences showing the deletion signature region (vertical box) in VACV-TO isolates and several VACV strains isolated during bovine vaccinia outbreaks. Arrow indicates polymorphism site in the hemagglutinin amino acid sequences between VACV-TO_CA and VACV-TO_AC. Alignments were made by using ClustalW (www.ncbi.nlm.nih.gov/pmc/articles/PMC308517) and MEGA version 3.1 software (www.megasoftware.net). HSPV, horsepoxvirus.