Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 16, Number 8—August 2010

Bat Coronaviruses and Experimental Infection of Bats, the Philippines

Shumpei Watanabe, Joseph S. Masangkay, Noriyo Nagata, Shigeru Morikawa, Tetsuya Mizutani, Shuetsu Fukushi, Phillip Alviola, Tsutomu Omatsu, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Shumpei Tsuda, Maiko Endoh, Kentaro Kato, Yukinobu Tohya, Shigeru Kyuwa, Yasuhiro Yoshikawa, and Hiroomi AkashiComments to Author 
Author affiliations: University of Tokyo, Tokyo, Japan (S. Watanabe, N. Ueda, K. Iha, S. Taniguchi, H. Fujii, S. Tsuda, M. Endoh, K. Kato, S. Kyuwa, Y. Yoshikawa, H. Akashi); University of the Philippines Los Baños, Laguna, the Philippines (J.S. Masangkay, P. Alviola); National Institute of Infectious Diseases, Tokyo (N. Nagata, S. Morikawa, T. Mizutani, S. Fukushi, T. Omatsu); Nihon University, Kanagawa, Japan (Y. Tohya)

Main Article

Table 4

Results of nested and quantitative RT-PCRs of cDNA from bat samples, the Philippines*

Bat Liver Kidney Lung Spleen Brain Small intestine Large intestine Serum
C + (ND)
D + (ND) + (ND) + (ND) + (6.57 × 104)† + (ND)
Intestine section
F + (ND) + (ND)
G + (ND) + (ND) + (ND) + 
(5.91 × 104)

*cDNA was synthesized from bat samples obtained after experimental infection (second passage of the group 2d coronavirus in Leschenault rousette bats). Values are copies per milligram. Virus load was quantified by real-time PCR. RT-PCR,reverse transcription–PCR; –, virus RNA not detected; +, virus RNA detected; ND, not detected by real-time PCR in RT-PCR–positive samples.
†Result of nested PCR.

Main Article

Page created: March 30, 2011
Page updated: March 30, 2011
Page reviewed: March 30, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.