Volume 17, Number 10—October 2011
Letter
Novel Hepatitis E Virus Genotype in Norway Rats, Germany
Wen Zhang, Quan Shen, Xiuguo Hua, and Li Cui
Table
Primers used in study (1) of amplification of complete genome sequence of rat HEV from rat sample 63, Germany*
| Designation | Binding position | Sequence† (5′ → 3′) |
|---|---|---|
| rHEV-RACEn‡ | 307–284 | GTGCTCATTAATAGATCGAGGGTG |
| rHEV-RACE‡ | 336–313 | GGAAGAAAACATCTGTGAATGACA |
| HEV-100s | 78–102 | CGGCCAATTCCGCCYTGGSRAATGC |
| rHEV-900as | 905–881 | TATGCCCGCCCGCACTAAACAAACT |
| HEV-800s | 776–801 | GTGCGGGCCATTGGCTGYCAYTTTGT |
| rHEV3300as | 3099–3074 | AGCCGCCATTCTGTTGGCTCCAGATT |
| rat5-s | 2921–2943 | CGCCGGGTTGTGATKGAYGAGGC |
| rHEV-td1-as | 4062–4037 | GAAATGCCCTGCCCGACCTTGCCATG |
| HEV-cs | 3977–3999 | TCGCGCATCACMTTYTTCCARAA |
| HEV-cas | 4446–4424 | GCCATGTTCCAGACDGTRTTCCA |
| Rat HEV-inv-s | 4301–4322 | GGGGCRCCYGAGTGGATGTGGA |
| 63-5400as | 5449–5428 | CTCAGTCGCCATGATATGCGTA |
| #8-ORF2-s | 5399–5421 | CCCTTACTGCCTYTKCAGGAYGG |
| #8-ORF2-as | 5604–5582 | GTGGAAGTGATGGAATTCATRTC |
| 63-5500s§ | 5555–5576 | CAATCCACAACAGTCCCCACGT |
*HEV, hepatitis E virus; RACE, rapid amplification of cDNA ends; ORF, open reading frame.
†D = A + G + T; K = G + T ; M = A + C; R = A + G; S = G + C; Y = C + T.
‡Primers were used for 5′-RACE.
§Primer was used for 3′-RACE.
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