Volume 17, Number 10—October 2011
Letter
Novel Hepatitis E Virus Genotype in Norway Rats, Germany
Table
Primers used in study (1) of amplification of complete genome sequence of rat HEV from rat sample 63, Germany*
Designation | Binding position | Sequence† (5′ → 3′) |
---|---|---|
rHEV-RACEn‡ | 307–284 | GTGCTCATTAATAGATCGAGGGTG |
rHEV-RACE‡ | 336–313 | GGAAGAAAACATCTGTGAATGACA |
HEV-100s | 78–102 | CGGCCAATTCCGCCYTGGSRAATGC |
rHEV-900as | 905–881 | TATGCCCGCCCGCACTAAACAAACT |
HEV-800s | 776–801 | GTGCGGGCCATTGGCTGYCAYTTTGT |
rHEV3300as | 3099–3074 | AGCCGCCATTCTGTTGGCTCCAGATT |
rat5-s | 2921–2943 | CGCCGGGTTGTGATKGAYGAGGC |
rHEV-td1-as | 4062–4037 | GAAATGCCCTGCCCGACCTTGCCATG |
HEV-cs | 3977–3999 | TCGCGCATCACMTTYTTCCARAA |
HEV-cas | 4446–4424 | GCCATGTTCCAGACDGTRTTCCA |
Rat HEV-inv-s | 4301–4322 | GGGGCRCCYGAGTGGATGTGGA |
63-5400as | 5449–5428 | CTCAGTCGCCATGATATGCGTA |
#8-ORF2-s | 5399–5421 | CCCTTACTGCCTYTKCAGGAYGG |
#8-ORF2-as | 5604–5582 | GTGGAAGTGATGGAATTCATRTC |
63-5500s§ | 5555–5576 | CAATCCACAACAGTCCCCACGT |
*HEV, hepatitis E virus; RACE, rapid amplification of cDNA ends; ORF, open reading frame.
†D = A + G + T; K = G + T ; M = A + C; R = A + G; S = G + C; Y = C + T.
‡Primers were used for 5′-RACE.
§Primer was used for 3′-RACE.