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Volume 17, Number 10—October 2011
Dispatch

Tembusu Virus in Ducks, China

Zhenzhen Cao, Cun Zhang, Yuehuan Liu, Weicheng Ye, Jingwen Han, Guoming Ma, Dongdong Zhang, Feng Xu, Xuhui Gao, Yi Tang, Shaohua Shi, Chunhe Wan, Chen Zhang, Bin He, Mengjie Yang, Xinhao Lu, Yu Huang, Youxiang Diao, Xuejun Ma, and Dabing ZhangComments to Author 
Author affiliations: Key Laboratory of Zoonosis of Ministry of Agriculture, Beijing, People’s Republic of China (Z. Cao, G. Ma, Dongdong Zhang, Dabing Zhang); China Agricultural University, Beijing (Z. Cao, G. Ma, Dongdong Zhang, Dabing Zhang); Zhejiang Academy of Agricultural Sciences, Hangzhou, People’s Republic of China (Cun Zhang, W. Ye); Beijing Academy of Agriculture and Forestry Sciences, Beijing (Y. Liu, J. Han); Beijing University of Agriculture, Beijing (F. Xu); Shandong Agricultural University, Taian, People’s Republic of China (X. Gao, Y. Tang, Y. Diao); Fujian Academy of Agricultural Sciences, Fuzhou, People’s Republic of China (S. Shi, C. Wan, Y. Huang); Chinese Center for Disease Control and Prevention, Beijing (Chen Zhang, B. He, M. Yang, X. Ma); Yuyao Municipal Institute of Poultry Disease, Yuyao, People’s Republic of China (X. Lu)

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Figure 2

Phylogenetic analysis of isolate YY5 (in boldface) from an ill Shaoxing duck in the People’s Republic of China and selected other flaviviruses obtained by using an ≈1-kb nt sequence in the nonstructural 5 genomic region. The tree was constructed by the neighbor-joining method of MEGA (7). Numbers at nodes indicate bootstrap percentages obtained after 1,000 replicates; only bootstrap values >70% are shown. Scale bar indicates genetic distance. The sequences used in the phylogenetic analysis ar

Figure 2. Phylogenetic analysis of isolate YY5 (in boldface) from an ill Shaoxing duck in the People’s Republic of China and selected other flaviviruses obtained by using an ≈1-kb nt sequence in the nonstructural 5 genomic region. The tree was constructed by the neighbor-joining method of MEGA (7). Numbers at nodes indicate bootstrap percentages obtained after 1,000 replicates; only bootstrap values >70% are shown. Scale bar indicates genetic distance. The sequences used in the phylogenetic analysis are as follows: AF013374, Gadgets Gully virus (GGYV); AF013385, Kyasanur Forest disease virus (KFDV); M86650, Langat virus (LGTV); Y07863, louping ill virus (LIV); AF013393, Omsk hemorrhagic fever virus (OHFV); L06436, Powassan virus (POWV); AF013398, Royal Farm virus (RFV); U27495, tick-borne encephalitis virus (TBEV); AF013380, Kadam virus (KADV); AF013386, Meaban virus (MEAV); AF013403, Saumarez Reef virus (SREV); AF013410, Tyuleniy virus (TYUV); AF013362, Aroa virus (AROAV); NC_001477, dengue virus 1 (DENV-1); AF013382, Kedougou virus (KEDV); AF013367, Cacipacore virus (CPCV); U15763, Japanese encephalitis virus (JEV); AF013384, Koutango virus (KOUV); AF013360, Alfuy virus (ALFV); AF013389, Murray Valley encephalitis virus (MVEV); AF013416, St. Louis encephalitis virus (SLEV); AF013412, Usutu virus (USUV); M12294, West Nile virus (WNV); D00246, Kunjin virus (KUNV); AF013413, Yaounde virus (YAOV); AF013383, Kokobera virus (KOKV); AF013363, Bagaza virus (BAGV); AF013376, Ilheus virus (ILHV); AF013397, Rocio virus (ROCV); AF013377, Israel turkey meningoencephalomyelitis virus (ITV); AF013392, Ntaya virus (NTAV); AF013408, Tembusu virus strain MM1775 (TMUV-MM1775); AF013409, TMUV strain THCAr (TMUV-THCAr); AB026994, TMUV strain Sitiawan (TMUV-Sitiawan); AF013415, Zika virus (ZIKV); AF013406, Spondweni virus (SPOV); L40951, Banzi virus (BANV); AF013364, Bouboui virus (BOUV); AF013372, Edge Hill virus (EHV); AF013378, Jugra virus (JUGV); AF013400, Saboya virus (SABV); AF013404, Sepik virus (SEPV); AF013411, Uganda S virus (UGSV); EU707555, Wesselsbron virus (WESSV); X03700, yellow fever virus (YFV); AF013373, Entebbe bat virus (ENTV); AF013414, Yokose virus (YOKV); AF013361, Apoi virus (APOIV); AF013370, Cowbone Ridge virus (CRV); AF013379, Jutiapa virus (JUTV); AF013387, Modoc virus (MODV); AF013401, Sal Vieja virus (SVV); AF013402, San Perlita virus (SPV); AF013365, Bukalasa bat virus (BBV); AF013368, Carey Island virus (CIV); AF013371, Dakar bat virus (DBV); AF013388, Montana myotis leukoencephalitis virus (MMLV); AF013394, Phnom Penh bat virus (PPBV); AF013396, Rio Bravo virus (RBV); M91671, cell fusing agent virus (CFAV); NC_003996, Tamana bat virus (TABV); and EU159426, Nounané virus (NOUV). The nucleotide sequence of isolate YY5 used in the phylogenetic analysis has been deposited in GenBank under accession no. HQ641390.

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References
  1. Dougherty  E III, Price  JI. Eastern encephalitis in white Pekin ducklings on Long Island. Avian Dis. 1960;4:24758. DOI
  2. Linssen  B, Kinney  RM, Aguilar  P, Russell  KL, Watts  DM, Kaaden  O, Development of reverse transcription-PCR assays specific for detection of equine encephalitis viruses. J Clin Microbiol. 2000;38:152735.PubMed
  3. Bondre  VP, Sapkal  GN, Yergolkar  PN, Fulmali  PV, Sankararaman  V, Ayachit  VM, Genetic characterization of Bagaza virus (BAGV) isolated in India and evidence of anti-BAGV antibodies in sera collected from encephalitis patients. J Gen Virol. 2009;90:26449. DOIPubMed
  4. Baillie  GJ, Kolokotronis  SO, Waltari  E, Maffei  JG, Kramer  LD, Perkins  SL. Phylogenetic and evolutionary analyses of St. Louis encephalitis virus genomes. Mol Phylogenet Evol. 2008;47:71728. DOIPubMed
  5. Bakonyi  T, Gould  EA, Kolodziejek  J, Weissenböck  H, Nowotny  N. Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001: comparison with the South African strain SAAR-1776 and other flaviviruses. Virology. 2004;328:30110. DOIPubMed
  6. Kuno  G, Chang  GJJ, Tsuchiya  KR, Karabatsos  N, Cropp  CB. Phylogeny of the genus Flavivirus. J Virol. 1998;72:7383.PubMed
  7. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:15969. DOIPubMed
  8. Kono  Y, Tsukamoto  K, Hamid  MA, Darus  A, Lian  TC, Sam  LS, Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus. Am J Trop Med Hyg. 2000;63:94101.PubMed

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1These authors contributed equally to this article.

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