Volume 17, Number 10—October 2011
Perspective
Global Spread of Carbapenemase-producing Enterobacteriaceae
Table 3
Oligonucleotides used for screening of main carbapenemase genes in Enterobacteriaceae*
Primer | Sequence, 5′ → 3′ | Gene | Product size, bp |
---|---|---|---|
IMP-F | GGAATAGAGTGGCTTAAYTC | blaIMP | 232 |
IMP-R | TCGGTTTAAYAAAACAACCACC | ||
VIM-F | GATGGTGTTTGGTCGCATA | blaVIM | 390 |
VIM-R | CGAATGCGCAGCACCAG | ||
OXA-48-F | GCGTGGTTAAGGATGAACAC | blaOXA-48 | 438 |
OXA-48-R | CATCAAGTTCAACCCAACCG | ||
NDM-F | GGTTTGGCGATCTGGTTTTC | blaNDM | 621 |
NDM-R | CGGAATGGCTCATCACGATC | ||
KPC-Fm | CGTCTAGTTCTGCTGTCTTG | blaKPC | 798 |
KPC-Rm | CTTGTCATCCTTGTTAGGCG |
*A detailed technique for PCR amplification has been reported by Poirel et al. (34). VIM, Verona integron–encoded metallo-β-lactamase; OXA, oxacillinase; NDM, New Delhi metallo-β-lactamase-1; KPC, Klebsiella pneumoniae carbapenemase. *A detailed technique for PCR amplification has been reported by Poirel et al. (34). VIM, Verona integron–encoded metallo-β-lactamase; OXA, oxacillinase; NDM, New Delhi metallo-β-lactamase-1; KPC, Klebsiella pneumoniae carbapenemase. |
*A detailed technique for PCR amplification has been reported by Poirel et al. (34). VIM, Verona integron–encoded metallo-β-lactamase; OXA, oxacillinase; NDM, New Delhi metallo-β-lactamase-1; KPC, Klebsiella pneumoniae carbapenemase.
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