Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 17, Number 12—December 2011
Research

Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

Steve Ahuka-Mundeke, Ahidjo Ayouba, Placide Mbala-Kingebeni, Florian Liegeois, Amandine Esteban, Octavie Lunguya-Metila, Didace Demba, Guy Bilulu, Valentin Mbenzo-Abokome, Bila-Isia Inogwabini, Jean-Jacques Muyembe-Tamfum, Eric Delaporte, and Martine PeetersComments to Author 
Author affiliations: University of Montpellier, Montpellier, France (S. Ahuka-Mundeke, A. Ayouba, F. Liegeois, A. Esteban, E. Delaporte, M. Peeters); Institut National de Recherche Biomédicales, Kinshasa, Democratic Republic of Congo (S. Ahuka-Mundeke, P. Mbala-Kingebeni, O. Lunguya-Metila, J.-J. Muyembe-Tamfum); Cliniques Universitaires de Kinshasa, Kinshasa (S. Ahuka-Mundeke, P. Mbala-Kingebeni, O. Lunguya-Metila, J.-J. Muyembe-Tamfum); Zone de Santé de Kole, Sankuru, Kasai Oriental, Democratic Republic of Congo (D. Demba, G. Bilulu); World Wildlife Fund For Nature, Kinshasa (V. Mbenzo-Abokome, B-I. Inogwabini)

Main Article

Table 2

Amino acid sequences of the 34 SIV/HIV peptides used to develop the xMAP gp41 and V3-loop multiplex Luminex assay

Peptide SIV lineage gp41 peptide sequences V3-loop peptide sequences
HIV-1 M HIV1/SIVcpz/gor LAVERYLKDQQLLGIWGCSGKLIC NNTRKSVRIGPGQAFYATGDIIGDIRQAYC
HIV-1 O HIV1/SIVcpz/gor LALGTLIQNQQLLNLWGCKGKLIC NLTVQEIKIGPMAWYSMGLAAGNGSRAYC
HIV-1 N HIV1/SIVcpz/gor LAIGRYLRDQQILSLWGCSGKTIC NNTGGQVGIGPAMTFYNIGKIVGDIRKAYC
SIVcpzPts HIV1/SIVcpz/gor LAVEKYLRDQQLLSLWGCADKVTC NRTVRNLQIGPGMTFYNVEIATGDTRKAFC
SIVcpzPtt HIV1/SIVcpz/gor LAVERYLQDQQILGLWGCSGKAVC NNTRGEVQIGPGMTFYNIENVVGDTRSAYC
SIVgor HIV1/SIVcpz/gor LAIETYLRDQQLLGLWGCTGKLIC NNTRGQIQIGPMTIYNSERIIGNTRKAYC
HIV-2/SIVsmm HIV-2/SIVsmm TAIEKYLKDQAQLNSWGCAFRQVC GNKTVVPITLMSGLVFMSQPINKRPRQAWC
SIVrcm SIVrcm TAIEKYLADQSLLNTFGCAWRQVC SNRTVKGISLAIGVFISLRVEKRPKGAWC
SIVagm SIVagm TALEKYLEDQARLNAWGCAWKQVC GNKTVLPVTIMAGLVFHSQKYNTLLRQAWC
SIVgsn SIVgsn/mus/mon† SSLEKYLRDQTILQAWGCANRPIC GNKTIRNLQIGAGMTFYSQVIVGGNTRKAYC
SIVmus SIVgsn/mus/mon† TALEKFVKDQAILNLWGCANRQIC †
SIVmon SIVgsn/mus/mon† TAVEKFIKDQTLLNAWGCANKAVC †
SIVdeb SIVdeb TAIEKYLKDQAKLNEWGCAFKQIC GNKTYRAVHMATGLSFYTTFIPRLRIKRAHC
SIVtal SIVtal‡ TALEKYLEDQAKLNSWGCAWKQIC RTIKDLQIAAGLMFHSQIIAGKDLKRAY
SIVsyk SIVsyk TALETYLRDQAIMSNWGCAFKQIC GNESIKNIQLAAGYFLPVIQGKLKTGRDAKRAFC
SIVlho SIVlho/sun TAIEEYLKDQALLASWGCQWKQVC GNRSEVSTISSTGLLFYYGLEHGSRLRLAQC
SIVmnd-2 SIVmnd TALEDYVADQSRLAVWGCSFSQVC GNRSVVSTPSATGLLFYHLGPGKNLKKGMC
SIVwrc SIVwrc SAIEGFLEDQLKLKQWGCELTQVC GNRSVVSVNSASGLIYYAGLEPHRNIRKGLC
SIVcol SIVcol‡ ATIEGYLEEQAKLASIGCANMQIC GNSSHRNLNTANGAKFYYELIPYSKGIYGRC

*SIV, simian immunodeficiency virus.
†V3 amino acid sequences of SIVgsn, SIVmus, and SIVmon were identical, and only the SIVgsn above the V3-loop peptide was synthesized.
‡Synthesis of SIVcol gp41 and SIVtal V3-loop peptides was unsuccessful.

Main Article

Page created: November 30, 2011
Page updated: November 30, 2011
Page reviewed: November 30, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external