Volume 17, Number 2—February 2011
Research
Possible Increased Pathogenicity of Pandemic (H1N1) 2009 Influenza Virus upon Reassortment
Eefje J.A. Schrauwen, Sander Herfst, Salin Chutinimitkul, Theo M. Bestebroer, Guus F. Rimmelzwaan, Albert D.M.E. Osterhaus, Thijs Kuiken, and Ron A.M. Fouchier
Table 2
Virus antigen expression and severity of lesion in different tissues of the lung of 3 ferrets inoculated with pandemic (H1N1) 2009 or reassortant pandemic (H1N1) 2009 viruses*
| Virus |
Cumulative score per tissue |
||||||||||
| Bronchial surface epithelium |
Bronchial submucosal epithelium |
Bronchiolar epithelium |
Alveolar epithelium |
||||||||
| IHC |
H&E |
IHC |
H&E |
IHC |
H&E |
IHC |
H&E |
||||
| Pandemic (H1N1) 2009 | 0, 0, 0† | 0, 0, 0 | 0, 0, 1 | 0, 0, 1 | 0, 0, 1 | 0, 0, 1 | 0, 0, 0 | 0, 0, 0 | |||
| Pandemic (H1N1) 2009–seasonal influenza A (H1N1) PB2PA | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | |||
| Pandemic (H1N1) 2009–seasonal influenza A (H1N1) PB2 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | 0, 0, 0 | |||
| Pandemic (H1N1) 2009–seasonal influenza A (H3N2) PB1NA | 1, 1, 0 | 0, 1, 0 | 2, 1, 0 | 1, 1, 0 | 2, 1, 0 | 1, 1, 0 | 1, 1, 0 | 1, 1, 1 | |||
| Pandemic (H1N1) 2009–seasonal influenza A (H3N2) NA | 1, 1, 1 | 0, 1, 1 | 1, 0, 3 | 1, 0, 3 | 2, 2, 3 | 2, 2, 3 | 1, 0, 1 | 1, 1, 1 | |||
*IHC, immunohistochemistry to detected virus antigen expression; H&E, hematoxylin and eosin staining to analyze severity of inflammation; PB, basic polymerase; PA, acidic polymerase; NA, neuraminidase.
†Individual scores (as indicated in the Methods section) for 3 ferrets are listed.
Page created: July 13, 2011
Page updated: July 13, 2011
Page reviewed: July 13, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.