Volume 17, Number 4—April 2011
Dispatch
Molecular Discrimination of Sheep Bovine Spongiform Encephalopathy from Scrapie
Laura Pirisinu, Sergio Migliore, Michele Angelo Di Bari, Elena Esposito, Thierry Baron, Claudia D’Agostino, Luigi De Grossi, Gabriele Vaccari, Umberto Agrimi, and Romolo Nonno
Table 2
Transmissible spongiform encephalopathy samples analyzed by discriminatory Western blot*
| Source | Identification no. | PrP genotype† | Blot lane |
|---|---|---|---|
| Natural isolates | |||
| Scrapie | ES16/10/10 | ARQ/ARQ | Sc1 |
| ES16/10/11 | ARQ/ARQ | Sc2 | |
| ES16/10/12 | ARQ/ARQ | Sc3 | |
| ES12/10/1 | ARQ/ARQ | ||
| ES12/10/2 | ARQ/ARQ | ||
| ES12/10/3 | ARQ/ARQ | Sc4 | |
| CH1641-like | 99–454 | VRQ/VRQ | Ch2 |
| 99–321 | VRQ/VRQ | Ch4 | |
| TR316211 | ARQ/ARQ | Ch3 | |
| Goat BSE |
CH636 |
Bs6 |
|
| Experimental samples | |||
| CH1641 | 241/74 | AxQ/AxQ | Ch1 |
| Sheep BSE | 301/16‡ | ARQ/ARQ | Bs1 |
| 301/44‡ | ARQ/ARQ | ||
| 302/87§ | ARQ/ARQ | Bs3 | |
| 302/130§ | ARQ/ARQ | Bs4 | |
| 302/64§ | ARQ/AHQ | Bs5 | |
| 302/90§ | ARQ/ARQ | Bs2 | |
*Blot lanes shown in Figure 1. PrP, prion protein; BSE, bovine spongiform encephalopathy.
†Amino acids at codons 136, 154 and 171.
‡Intracerebral transmission.
§Oral transmission.
Page created: July 25, 2011
Page updated: July 25, 2011
Page reviewed: July 25, 2011
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