Volume 17, Number 6—June 2011
Dispatch
Increasing Ceftriaxone Resistance in Salmonellae, Taiwan
Table 2
Characteristics of conjugative blaCMY-2-harboring IncI1 plasmids derived in this study and comparison of pMLST patterns with similar plasmids published previously*
IncI1 plasmid |
Salmonella enterica serotype or Escherichia coli |
Susceptibility profile† |
Country |
Year of isolation |
pMLST‡ | ST§ |
Clonal complex |
Reference |
||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
repI1 |
ardA |
trbA |
sogS |
pilL |
||||||||
pSC-B134 | Choleraesuis | R/S/S | Taiwan | 2007 | 1 | 1 | 15 | 9 | 3 | 51 | NA | This study |
pSC-B136 | Choleraesuis | R/R/S | Taiwan | 2008 | 1 | 4 | 15 | 11 | 2 | 52 | NA | This study |
pSB-5 | Typhimurium variant Copenhagen | R/R/R | Taiwan | 2010 | 2 | 1 | 15 | 11 | 2 | 53 | NA | This study |
pSB28 | Typhimurium | S/S/S | Taiwan | 2010 | 1 | 4 | 5 | 11 | 2 | 54 | NA | This study |
pSB151 | Typhimurium | S/S/S | Taiwan | 2010 | 4 | 5 | 15 | 11 | 3 | 55 | NA | This study |
pSB193 | Typhimurium | S/S/S | Taiwan | 2010 | 1 | 4 | 5 | 11 | 2 | 54 | NA | This study |
pSB105 | Agona | R/S/S | Taiwan | 2010 | 2 | 1 | 15 | 11 | 3 | 56 | NA | This study |
pSD166 | Enteritidis | R/S/S | Taiwan | 2010 | 2 | 1 | 15 | 11 | 3 | 56 | NA | This study |
398T | E. coli | NA | Italy | 2006 | 1 | 2 | 3 | 2 | 1 | 2 | CC-2 | (12) |
05–1909 | Heidelberg | NA | Canada | 2005 | 1 | 2 | 3 | 2 | 1 | 2 | CC-2 | (13) |
1358T | Thompson | NA | USA | 1996 | 1 | 3 | 3 | 4 | 1 | 4 | CC-12 | (12) |
DH-20406 | Heidelberg | NA | USA | 2004 | 1 | 4 | 3 | 4 | 1 | 12 | CC-12 | (14) |
06–3048 | 4,5,12:i:– | NA | Canada | 2006 | 1 | 4 | 3 | 4 | 1 | 12 | CC-12 | (13) |
06–3539 | Agona | NA | Canada | 2006 | 1 | 4 | 3 | 4 | 1 | 12 | CC-12 | (13) |
N07–0084 | E. coli | NA | Canada | 2005 | 1 | 4 | 3 | 4 | 1 | 12 | CC-12 | (13) |
05–2835 | Heidelberg | NA | Canada | 2005 | 1 | 4 | 3 | 4 | 1 | 12 | CC-12 | (13) |
06–3985 | Litchfield | NA | Canada | 2006 | 1 | 4 | 3 | 4 | 1 | 12 | CC-12 | (13) |
05–5567 | Typhimurium | NA | Canada | 2005 | 1 | 4 | 3 | 4 | 1 | 12 | CC-12 | (13) |
N06–523 | E. coli | NA | Canada | 2006 | 1 | 4 | 3 | 2 | 1 | 18 | CC-2/ CC-12 | (13) |
N06–0537 | E. coli | NA | Canada | 2006 | 1 | 3 | 3 | 4 | 3 | 19 | CC-12 | (13) |
05–6117 | 4,5,12:1:– | NA | Canada | 2006 | 1 | 1 | 3 | 9 | 1 | 20 | NA | (13) |
N07–0093 | E. coli | NA | Canada | 2005 | 1 | 1 | 3 | 9 | 1 | 20 | NA | (13) |
06–0753 | Heidelberg | NA | Canada | 2006 | 1 | 2 | 11 | 3 | 3 | 21 | CC-5 | (13) |
N07–0079 | E. coli | NA | Canada | 2005 | 1 | 6 | 3 | 4 | 1 | 22 | CC-12 | (13) |
N07–0081 | E. coli | NA | Canada | 2005 | 1 | 2 | 3 | 1 | 1 | 23 | CC-2 | (13) |
*pMLST, plasmid multilocus sequence typing; ST, sequence type; R, resistant; S, susceptible; NA, not applicable.
†Antimicrobial drug susceptibility to chloramphenicol, trimethoprim/sulfamethoxazole, and quinolones (nalidixic acid and ciprofloxacin). The transconjugants were also resistant to ampicillin and ceftriaxone.
‡pMLST results were compared to those published in the plasmid MLST website (http://pubmlst.org/plasmid).
§STs were designated according to the different combinations of allele variants observed among the IncI1 plasmids.
References
- Vugia DJ, Samuel M, Farley MM, Marcus R, Shiferaw B, Shallow S, Invasive Salmonella infections in the United States, FoodNet, 1996–1999: incidence, serotype distribution, and outcome. Clin Infect Dis. 2004;38(Suppl 3):S149–56. DOIPubMedGoogle Scholar
- Su LH, Chiu CH, Chu C, Ou JT. Antimicrobial resistance in nontyphoid Salmonella serotypes: a global challenge. Clin Infect Dis. 2004;39:546–51. DOIPubMedGoogle Scholar
- Chiu CH, Wu TL, Su LH, Chu C, Chia JH, Kuo AJ, The emergence in Taiwan of fluoroquinolone resistance in Salmonella enterica serotype choleraesuis. N Engl J Med. 2002;346:413–9. DOIPubMedGoogle Scholar
- Chiu CH, Su LH, Chu C, Chia JH, Wu TL, Lin TY, Isolation of Salmonella enterica serotype choleraesuis resistant to ceftriaxone and ciprofloxacin. Lancet. 2004;363:1285–6. DOIPubMedGoogle Scholar
- Ye J, Su LH, Chen CL, Hu S, Wang J, Yu J, Complete nucleotide sequence of pSC138, the multidrug resistance plasmid of Salmonella enterica serotype Choleraesuis SC-B67. Plasmid. 2011;65:132–40. DOIPubMedGoogle Scholar
- Su LH, Chen HL, Chia JH, Liu SY, Chu C, Wu TL, Distribution of a transposon-like element carrying blaCMY-2 among Salmonella and other Enterobacteriaceae. J Antimicrob Chemother. 2006;57:424–9. DOIPubMedGoogle Scholar
- Su LH, Leu HS, Chiu YP, Chia JH, Kuo AJ, Sun CF, Molecular investigation of two clusters of nosocomial bacteraemia caused by multiresistant Klebsiella pneumoniae using pulsed-field gel electrophoresis and infrequent-restriction-site PCR. J Hosp Infect. 2000;46:110–7. DOIPubMedGoogle Scholar
- Kado CI, Liu ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981;145:1365–73.PubMedGoogle Scholar
- Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975;98:503–17. DOIPubMedGoogle Scholar
- Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005;63:219–28. DOIPubMedGoogle Scholar
- Jacoby GA, Han P. Detection of extended-spectrum β-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli. J Clin Microbiol. 1996;34:908–11.PubMedGoogle Scholar
- Cloeckaert A, Praud K, Lefevre M, Doublet B, Pardos M, Granier SA, IncI1 plasmid carrying extended-spectrum-β-lactamase gene blaCTX-M-1 in Salmonella enterica isolates from poultry and humans in France, 2003 to 2008. Antimicrob Agents Chemother. 2010;54:4484–6. DOIPubMedGoogle Scholar