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Volume 18, Number 1—January 2012

Serious Invasive Saffold Virus Infections in Children, 2009

Alex Christian Yde NielsenComments to Author , Blenda Böttiger, Jytte Banner, Thomas Hoffmann, and Lars P. Nielsen
Author affiliations: University of Southern Denmark, Odense, Denmark (A.C.Y. Nielsen); Statens Serum Institut, Copenhagen, Denmark (A.C.Y. Nielsen, B. Böttiger, L.P. Nielsen); University of Aarhus, Aarhus, Denmark (J. Banner); Hvidovre Hospital, Copenhagen (T. Hoffmann)

Main Article


Primers and probe used for diagnostic PCR and primers used to sequence Saffold virus, 2009*

Assay, primer Type Sequence, 5′ → 3′
Diagnostic RT-PCR
Saffold F Forward primer CTA WCA TGC CTC CCC GAT T
Saffold R Reverse primer GYT TAG ACC GGG GGA ACC
Saffold Probe Probe TTT CTG CCC TGC TGG GCG G
VP2 typing
SAFV VP2 OR Outer reverse primer CCR TTR AAN ACS GGY TTN AC
SAFV VP2 IF Inner forward primer CGG CYA YAA ACA CKC AAT C
SAFV VP2 IR Inner reverse primer TTD GCR TGY TGN GTC CA
VPI typing, type 2 specific
SAFV-2 VP1 OF1 Outer forward primer 1 GCA GAA AAA GGA AAG GTT GC
SAFV-2 VP1 OF2 Outer forward primer 2 GCA GAA AAA GGC AAA GTT GC
SAFV-2 VP1 OR Outer reverse primer TCT TGG RCA AAA CAC TCT CA
SAFV-2 VP1 IF Inner forward primer CYA TAG CTC TTC CTG AAA AYC A
SAFV-2 VP1 IR Inner reverse primer TGR ACC GAA AAY CTG TCT GC

*RT-PCR, real-time reverse transcription PCR; SAFV, Saffold virus: VP, viral protein.

Main Article

Page created: December 16, 2011
Page updated: December 16, 2011
Page reviewed: December 16, 2011
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