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Volume 18, Number 10—October 2012
Research

Nontuberculous Mycobacteria in Household Plumbing as Possible Cause of Chronic Rhinosinusitis

Wellington S. Tichenor1Comments to Author , Jennifer Thurlow1, Steven McNulty, Barbara A. Brown-Elliott, Richard J. Wallace, and Joseph O. Falkinham
Author affiliations: The Center for Allergy, Asthma and Sinusitis, New York, New York, USA (W.S. Tichenor, J. Thurlow); New York Medical College, Valhalla, New York (W.S. Tichenor); University of Texas Health Science Center, Tyler, Texas, USA (S. NcNulty, B.A. Brown-Elliott, R.J. Wallace, Jr.); Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (J.O. Falkinham III)

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Figure 2

Pulsed field gel electrophoresis (PFGE) of AseI digest patterns of patient and household isolates, New York, New York, USA, 2001–2011. A) Patient and household isolates. Lane 1, λ ladder; lane 2, patient no. 5 Mycobacterium avium isolate AG-P-1; lane 3, patient no. 5 household filter M. avium isolate AG-F-2–0-2; lane 4, patient no. 5 household filter M. avium isolate AG-F-2-I-1(environmental isolates in lanes 3 and 4 are indistinguishable; patient isolate in lane 2 considered clonal with 2 envir

Figure 2. . . . Pulsed-field gel electrophoresis (PFGE) of AseI digest patterns of patient and household isolates, New York, New York, USA, 2001–2011. A) Patient and household isolates. Lane 1, λ ladder; lane 2, patient no. 5 Mycobacterium avium isolate AG-P-1; lane 3, patient no. 5 household filter M. avium isolate AG-F-2-0-2; lane 4, patient no. 5 household filter M. avium isolate AG-F-2-I-1 (environmental isolates in lanes 3 and 4 are indistinguishable; patient isolate in lane 2 considered clonal with 2 environmental isolates [6 bands difference]); with digestion with XbaI, the 3 were considered closely related.); lane 5, patient no. 6 M. avium complex “X” cluster (MAC-X) isolate GG-P-1; lane 6, patient no. 6 household swab M. chimaera isolate GG-Sw-9–1 (despite overall similarity, isolates in lanes 5 and 6 belong to different species and differ by 10 bands and are therefore unrelated). B) Additional patient and isolate from the person’s household. Lane 1, patient no. 2 M. avium isolate BB-P-1; lane 2, patient no. 2 household water M. avium isolate BB-W-4–5; lane 3, λ ladder.

Main Article

1These authors contributed equally to this article.

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