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Volume 18, Number 12—December 2012
Dispatch

Variant Rabbit Hemorrhagic Disease Virus in Young Rabbits, Spain

Kevin P. Dalton, Inés Nicieza, Ana Balseiro, María A. Muguerza, Joan M. Rosell, Rosa Casais, Ángel L. Álvarez, and Francisco ParraComments to Author 
Author affiliations: Universidad de Oviedo Instituto Universitario de Biotecnología de Asturias, Oviedo, Spain (K.P. Dalton, I. Nicieza, A.L. Álvarez, F. Parra); Centro de Biotecnología Animal (SERIDA), Gijón, Spain (A. Balseiro, R. Casais); INTIA División ITG, Navarra, Spain (M.A. Muguerza); and Cunivet Service, Tarragona, Spain (J.M. Rosell)

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Figure 1

Evolutionary relationships of rabbit hemorrhagic disease virus (RHDV) and related viruses. A total of 38 nt sequences were analyzed: the isolate from this study, designated RHDV-N11 (GenBank accession no. JX133161); 18 classical RHDV and 12 RHDVa isolates; 6 rabbit calicivirus (RCV)–like isolates; and European brown hare syndrome virus (GenBank accession no. Z69620) as an outlier. Evolutionary history was inferred by using the neighbor-joining method; optimal tree with the sum of branch length =

Figure 1. . Evolutionary relationships of rabbit hemorrhagic disease virus (RHDV) and related viruses. A total of 38 nt sequences were analyzed: the isolate from this study, designated RHDV-N11 (GenBank accession no. JX133161); 18 classical RHDV and 12 RHDVa isolates; 6 rabbit calicivirus (RCV)–like isolates; and European brown hare syndrome virus (GenBank accession no. Z69620) as an outlier. Evolutionary history was inferred by using the neighbor-joining method; optimal tree with the sum of branch length = 1.33075964 is shown. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. Evolutionary distances were computed by using the p-distance method (14) and are in the units of the number of base differences per site. Codon positions included were 1st + 2nd + 3rd + Noncoding. All ambiguous positions were removed for each sequence pair; the final dataset consisted of 646 positions. Evolutionary analyses were conducted in MEGA5 (15). RHDV genetic groups are indicated. GenBank accession numbers of the sequences were: RCV-like strains: EU871528, GU368915, X96868, EF558587, AM268419, GQ166866; RHDV strains; AJ006019, AJ535094, Y15424, Y15426, Y15441, EF363035, Y15427, AF402614, RHU54983, AF295785, Z49271, L48547, Y15440, RHU49726, Z29514, M67473, X87607, FR823355; RHDVa strains: DQ069280, DQ280493, DQ069282, AY269825, DQ841708, DQ205345, AF258618, DQ069281, AJ969628, AF453761, EF558584, Y15442.

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