Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 18, Number 12—December 2012


Variant Rabbit Hemorrhagic Disease Virus in Young Rabbits, Spain

Kevin P. Dalton, Inés Nicieza, Ana Balseiro, María A. Muguerza, Joan M. Rosell, Rosa Casais, Ángel L. Álvarez, and Francisco ParraComments to Author 
Author affiliations: Universidad de Oviedo Instituto Universitario de Biotecnología de Asturias, Oviedo, Spain (K.P. Dalton, I. Nicieza, A.L. Álvarez, F. Parra); Centro de Biotecnología Animal (SERIDA), Gijón, Spain (A. Balseiro, R. Casais); INTIA División ITG, Navarra, Spain (M.A. Muguerza); and Cunivet Service, Tarragona, Spain (J.M. Rosell)

Main Article

Table 2

Immunohistochemical detection of RHDV-N11 in tissues from experimentally infected and control rabbits by using monoclonal antibody 6G2*

Rabbit no. and age Infection method Tissue
Liver Lung Kidney Spleen Intestine Heart
1. Adult (control) SC +/–
2. Young (control)† SC + ++ ++ + ++ +/–
3. Young SC
4. Adult‡ SC + + ++ +++ + +/–
5. Young‡ SC +++ + ++ +++ ++ +/–
6. Adult SC
7. Adult IN +
8. Young IN

*Young rabbits were 30 d of age on the day of experimental infection. Control rabbits (1 and 2) were each injected with 500 µL sterile PBS. RHDV, rabbit hemorrhagic disease virus; PBS, phosphate-buffered saline; SC, subcutaneous injection; IN, intranasal administration; –, not detected; +, ++, +++, increasing levels of antigen.
†Died 96 h after experimental infection of animals 3–8.
‡Died 48 h after experimental infection.

Main Article