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Volume 18, Number 7—July 2012


Transmission of Bordetella holmesii during Pertussis Outbreak, Japan

Hajime Kamiya, Nao Otsuka, Yuka Ando, Fumito Odaira, Shuji Yoshino, Kimiko Kawano, Hirokazu Takahashi, Toshihide Nishida, Yoshio Hidaka, Hiromi Toyoizumi-Ajisaka, Keigo Shibayama, Kazunari KamachiComments to Author , Tomimasa Sunagawa, Kiyosu Taniguchi, and Nobuhiko Okabe
Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (H. Kamiya, N. Otsuka, Y. Ando, F. Odaira, H. Toyoizumi-Ajisaka, K. Shibayama, K. Kamachi, T. Sunagawa, K. Taniguchi, N. Okabe); Miyazaki Prefectural Institute for Public Health and Environment, Miyazaki, Japan (S. Yoshino, K. Kawano); Takahashi Clinic, Miyazaki (H. Takahashi); and Nobeoka Public Health Center, Miyazaki (T. Nishida, Y. Hidaka)

Main Article

Table 1

Characteristics of Bordetella holmesii infection in 6 patients during pertussis outbreak, Japan, September 2010–April 2011*

Patient no. Age, y/sex Duration of cough, d† B. holmesii test results
recA real-time PCR (Ct)‡ Culture§
1 17/M 5 + (28.7) +
2 15/F 4 + (23.4) +
3 15/F >14 + (21.6) +
4 14/F 8 + (25.1) +
5 40/M 8 + (27.0) +
6 45/F 15 + (36.6)

*All patients had negative results for B. pertussis loop-mediated isothermal amplification and positive results for IS481 PCR. Ct, cycle threshold; –, negative; +, positive.
†At time of specimen collection.
‡Detection limit was a Ct value of 38.7, corresponding to 100 fg of DNA of B. holmesii ATCC51541.
§All strains were isolated from nasopharyngeal swab specimens.

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