Skip directly to page options Skip directly to A-Z link
Volume 18, Number 7—July 2012
Letter

Culicoids as Vectors of Schmallenberg Virus

Lasse Dam Rasmussen, Birgit Kristensen, Carsten Kirkeby, Thomas Bruun Rasmussen, Graham J. Belsham, René Bødker, and Anette BøtnerComments to Author 
Author affiliations: Technical University of Denmark, Kalvehave, Denmark (L.D. Rasmussen, T.B. Rasmussen, G.J. Belsham, A. Bøtner); Technical University of Denmark, Copenhagen, Denmark (B. Kristensen, C. Kirkeby, R. Bødker)

Main Article

Figure

RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse transcription quantitative PCRs (RT-qPCRs) for the Schmallenberg virus L segment, and the products were analyzed by agarose gel electrophoresis. Lanes 1–8, C. obsoletus group midge pools 1–8; lanes 9–10; negative and positive controls, respectively. Numbers below lanes are cycle threshold values from RT-qPCRs; –, no value. M, size marker. Amplicons (145 bp) from positive pools were extracted and sequenced.

Figure. . . RNA extracted from pools of Culicoides obsoletus group midges was tested in 1-step reverse transcription quantitative PCRs (RT-qPCRs) for the Schmallenberg virus large segment, and the products were analyzed by agarose gel electrophoresis. Lanes 1–8, C. obsoletus group midge pools 1–8; lanes 9–10; negative and positive controls, respectively. Numbers below lanes are cycle threshold values from RT-qPCRs; –, no value. M, size marker. Amplicons (145 bp) from positive pools were extracted and sequenced.

Download

Main Article

Page created: June 13, 2012
Page updated: June 13, 2012
Page reviewed: June 13, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external