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Volume 19, Number 10—October 2013


Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus

Chao Qiu1, Yang Huang1, Anli Zhang1, Di Tian1, Yanmin Wan1, Xiaoling Zhang, Wanju Zhang, Zhiyong Zhang, Zhenghong Yuan, Yunwen Hu, Xiaoyan Zhang, and Jianqing XuComments to Author 
Author affiliations: Shanghai Public Health Clinical Center, Shanghai, China (C. Qiu, A. Zhang, D. Tian, Y. Wan, Xiaoling Zhang, W. Zhang, Z. Zhang, Z. Yuan, Y. Hu, Xiaoyan Zhang, J. Xu); Fudan University, Shanghai (C. Qiu, Y. Huang, A. Zhang, Z. Yuan, Xiaoyan Zhang, J. Xu); Chinese Center for Disease Control and Prevention, Beijing, China (Xiaoyan Zhang, J. Xu)

Main Article


Comparison of HI and pseudovirus-based neutralization assay results for influenza A(H7N9) virus*

HI titer†
Pseudovirus IC50 titer
+ 12 2 13 1
0 50 4 46

*Values are no. samples. HI, hemagglutination inhibition; IC50, 50% inhibitory concentration.
†+ HI titer is >40; – HI titer is <40.
‡κ = 0.904; McNemar test, p = 0.5.
§κ = 0.788; McNemar test, p = 0.375.

Main Article

1These authors contributed equally to this article.