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Volume 19, Number 11—November 2013

Atypical Scrapie Prions from Sheep and Lack of Disease in Transgenic Mice Overexpressing Human Prion Protein

Jonathan D.F. WadsworthComments to Author , Susan Joiner, Jacqueline M. Linehan, Anne Balkema-Buschmann, John Spiropoulos, Marion M. Simmons, Peter C. Griffiths, Martin H. Groschup, James Hope, Sebastian Brandner, Emmanuel A. Asante, and John Collinge
Author affiliations: University College London, London, UK (J.D.F Wadsworth, S. Joiner, J.M. Linehan, S. Brandner, E.A. Asante, J. Collinge); Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany (A. Anne Balkema-Buschmann, M.H. Groschup); Animal Health and Veterinary Laboratories Agency, Addlestone, UK (J. Spiropoulos, M.M. Simmons, P.C. Griffiths, J. Hope)

Main Article

Table 1

Details of prion sources for ovine inocula*

Source code Brain region Prion agent Ovine PrP genotype‡
Transmission data†
Attack rate (incubation period) Reference
AHVLA/SE 1919/0077 Cerebral cortex Classical scrapie VRQ/VRQ tg338 mice§; 16/16 (64 ± 2 d) (21); code SE 1848/0005
AHVLA/SE 1919/0080 Cerebral cortex Classical scrapie ARQ/ARQ tg338 mice§ 12/13 (155 ± 4 d) (21); code SE 1848/0008
FLI 1/06 Caudal medulla Classical scrapie ARQ/ARQ ND NA
FLI 83/04 Caudal medulla Classical scrapie ARQ/ARQ ND NA
FLI 107/04 Caudal medulla Classical scrapie ARQ/ARQ ND NA
AHVLA/SE 1850/0001 Caudal medulla Atypical scrapie AHQ/AHQ tg338 mice§; 19/20 (210 ± 3 d) (22); code 1
AHVLA/SE 1850/0009 Caudal medulla Aypical scrapie ARR/ARR tg338 mice§; 19/19 (231 ± 6 d) (22); code 9
FLI S7/06 Caudal medulla Atypical scrapie AHQ/ARQ ND NA
FLI 14/06 Caudal medulla Atypical scrapie ARR/ARR ND NA
FLI 26/06 Caudal medulla Atypical scrapie AHQ/ARQ ND NA
AHVLA/SE 1929/0877 Caudal medulla Ovine BSE ARQ/ARQ RIII mice; 16/19 (422 ± 19 d)¶ Unpub.
AHVLA/SE1945/0032 Rostral medulla 2nd Passage ovine BSE ARQ/ARQ RIII mice; 18/20 (356 ± 9 d)¶ Unpub.

*PrP, prion protein; AHVLA, Animal Health and Veterinary Laboratories Agency; ND, not done; NA, not applicable; FLI, Friedrich-Loeffler-Institut; BSE, bovine spongiform encephalopathy; Unpub., unpublished.
†Reports attack rate (the no. of infected mice as a proportion of the no. of inoculated mice) and mean incubation period in days ± SEM or SD and the reference in which the data were first published with original inocula code.
‡Ovine PrP codon 136,154,171 genotype.
§Transgenic for the ovine Prnp VRQ allele on a mouse Prnp0/0 background. Overexpression 8- to 10-fold of normal ovine brain.
¶Mean incubation period in days ± SEM.

Main Article

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Page updated: October 31, 2013
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