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Volume 19, Number 11—November 2013
Research

Atypical Scrapie Prions from Sheep and Lack of Disease in Transgenic Mice Overexpressing Human Prion Protein

Jonathan D.F. WadsworthComments to Author , Susan Joiner, Jacqueline M. Linehan, Anne Balkema-Buschmann, John Spiropoulos, Marion M. Simmons, Peter C. Griffiths, Martin H. Groschup, James Hope, Sebastian Brandner, Emmanuel A. Asante, and John Collinge
Author affiliations: University College London, London, UK (J.D.F Wadsworth, S. Joiner, J.M. Linehan, S. Brandner, E.A. Asante, J. Collinge); Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany (A. Anne Balkema-Buschmann, M.H. Groschup); Animal Health and Veterinary Laboratories Agency, Addlestone, UK (J. Spiropoulos, M.M. Simmons, P.C. Griffiths, J. Hope)

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Table 1

Details of prion sources for ovine inocula*

Source code Brain region Prion agent Ovine PrP genotype‡
Transmission data†
Attack rate (incubation period) Reference
AHVLA/SE 1919/0077 Cerebral cortex Classical scrapie VRQ/VRQ tg338 mice§; 16/16 (64 ± 2 d) (21); code SE 1848/0005
AHVLA/SE 1919/0080 Cerebral cortex Classical scrapie ARQ/ARQ tg338 mice§ 12/13 (155 ± 4 d) (21); code SE 1848/0008
FLI 1/06 Caudal medulla Classical scrapie ARQ/ARQ ND NA
FLI 83/04 Caudal medulla Classical scrapie ARQ/ARQ ND NA
FLI 107/04 Caudal medulla Classical scrapie ARQ/ARQ ND NA
AHVLA/SE 1850/0001 Caudal medulla Atypical scrapie AHQ/AHQ tg338 mice§; 19/20 (210 ± 3 d) (22); code 1
AHVLA/SE 1850/0009 Caudal medulla Aypical scrapie ARR/ARR tg338 mice§; 19/19 (231 ± 6 d) (22); code 9
FLI S7/06 Caudal medulla Atypical scrapie AHQ/ARQ ND NA
FLI 14/06 Caudal medulla Atypical scrapie ARR/ARR ND NA
FLI 26/06 Caudal medulla Atypical scrapie AHQ/ARQ ND NA
AHVLA/SE 1929/0877 Caudal medulla Ovine BSE ARQ/ARQ RIII mice; 16/19 (422 ± 19 d)¶ Unpub.
AHVLA/SE1945/0032 Rostral medulla 2nd Passage ovine BSE ARQ/ARQ RIII mice; 18/20 (356 ± 9 d)¶ Unpub.

*PrP, prion protein; AHVLA, Animal Health and Veterinary Laboratories Agency; ND, not done; NA, not applicable; FLI, Friedrich-Loeffler-Institut; BSE, bovine spongiform encephalopathy; Unpub., unpublished.
†Reports attack rate (the no. of infected mice as a proportion of the no. of inoculated mice) and mean incubation period in days ± SEM or SD and the reference in which the data were first published with original inocula code.
‡Ovine PrP codon 136,154,171 genotype.
§Transgenic for the ovine Prnp VRQ allele on a mouse Prnp0/0 background. Overexpression 8- to 10-fold of normal ovine brain.
¶Mean incubation period in days ± SEM.

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Page created: October 31, 2013
Page updated: October 31, 2013
Page reviewed: October 31, 2013
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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