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Volume 19, Number 11—November 2013

Full Genome of Influenza A (H7N9) Virus Derived by Direct Sequencing without Culture

Xianwen Ren1, Fan Yang1, Yongfeng Hu1, Ting Zhang1, Liguo Liu1, Jie Dong, Lilian Sun, Yafang Zhu, Yan Xiao, Li Li, Jian Yang, Jianwei Wang, and Qi JinComments to Author 
Author affiliations: MOH Key Laboratory of Systems Biology of Pathogens, Beijing, China

Main Article

Table 2

Heterogeneous genomic positions of directly sequenced influenza A (H7N9) virus and its protein differences from other viruses*

Protein Heterogeneity, nucleotide position in gene sequence: nucleotides)† Amino acid position in protein sequence‡ Direct sequencing§ Culture§# A/Anhui/1/2013§ Consensus of isolate from humans§**
HA 330: C>T 110 F F F F
HA 360: C>T 120 L L L L
HA 696: T>A 232 V V V V
HA 724: C>T 242 H242Y H H H
HA 762: C>T 254 F F F F
HA 780: C>T 260 F F F F
HA 1441: C>T 481 H H H H
HA 65 M M R R
M2 10 L L P P
M2 24 D D E E
NA 275: C>T 92 S92L S S S
NA 323: C>T 108 S108L S S S
NA 408: C>T 136 I I I I
NA 40 S S G G
NA 300 V V I I
NA 340 I I N N
NP 321 S N N N
NP 858: C>T 286 A A A A
NP 583: G>A 195 R R R R
NP 1261: G = A 421 E421K E E E
NP 1260: C = T 420 F F F F
NP 371 M I M M
NS2 546: C>>T 55 L L L L
NS2 543: C>>T 54 D D D D
NS1 418: C>>T 140 R140W R R R
NS1 59 R H R R
NS2 107 P L L L
NS2 111 V Q Q Q
PA 174: C>A 58 G G G G
PA 1305:C>T 435 I I I I
PA 618 K K T T
PA 707 V F F F
PB1 200 I I V V
PB1 368 I I V V
PB1 454 L L P P
PB1 637 V V I I
PB1-F2 42 C C Y Y
PB1-F2 51 T T M M
PB1-F2 70 G E G G
PB1-F2 77 L L S S
PB2 534 S F S S
PB2 591 K K Q Q
PB2 627 E E K K

*HA, hemagglutinin; M, matrix; NA, neuraminidase; NP, nucleocapsid protein; NS, nonstructural; PA, polymerase acidic; PB,
polymerase basic.
†Position of first nucleotide = 1.
‡Position of first amino acid = 1.
§Types of amino acids.
#Virus cultured in chicken eggs.
**Thirteen influenza A (H7N9) viruses isolated from humans; data from Global Initiative on Sharing All Influenza Data.

Main Article

1These authors contributed equally to this article.

Page created: August 15, 2013
Page updated: October 29, 2013
Page reviewed: October 29, 2013
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