Volume 19, Number 4—April 2013
Research
Transmission of Hepatitis E Virus from Rabbits to Cynomolgus Macaques
Table
Comparison of the complete genome sequence of rabbit HEV passed in macaques with that of the inoculum*
Nucleotide position† | Genomic region | Nucleotide |
Amino acid |
|||
---|---|---|---|---|---|---|
CHN-BJ-R14‡ | rHEV-Cy1§ | Position† | Substitution | |||
614 | ORF1-MeT | C | T | 197 | Silent | |
957 | ORF1-Y | T | C | 311 | Thr to Ile | |
1667 | ORF1-PCP | T | C | 548 | Silent | |
1875 | ORF1 | T | C | 617 | Pro to Leu | |
2706 | ORF1-X | G | A | 894 | Asp to Gly | |
3553 | ORF1-Hel | A | T | 1176 | Silent | |
3571 | ORF1-Hel | C | T | 1182 | Silent | |
3859 | ORF1-RdRp | C | A | 1278 | Silent | |
3889 | ORF1-RdRp | C | T | 1288 | Silent | |
3972 | ORF1-RdRp | G | A | 1316 | Glu to Gly | |
4215 | ORF1-RdRp | C | T | 1397 | Leu to Pro | |
4285 | ORF1-RdRp | A | G | 1420 | Silent | |
4414 | ORF1-RdRp | T | C | 1463 | Silent | |
4427 | ORF1-RdRp | C | T | 1468 | Tyr to His | |
4882 | ORF1-RdRp | T | C | 1619 | Silent | |
5028 | ORF1-RdRp | T | C | 1668 | Ala to Val | |
5531 | ORF2 | C | T | 100 | Silent | |
ORF3 | 104 | Ala to Val | ||||
5713 | ORF2 | T | A | 161 | Ile to Asn |
*HEV, hepatitis E virus; ORF, open reading frame; Thr, Threonine; Ile, Isoleucine; Pro, proline; Leu, leucine; Asp, aspartic acid; Gly, glycine; Glu, glutamic acid; Tyr, tyrosine; His, histidine; Ala, alanine; Val, valine; Asn, asparagine.
†Nucleotide or amino acid position according to the rabbit HEV CHN-BJ-R14 strain.
‡CHN-BJ-R14, HEV isolate recovered from the rabbit HEV inoculum in this study.
§rHEV-Cy1, HEV isolate recovered from the fecal sample of 1 monkey at 3 wpi in this study.
¶Putative domains in ORF1. MeT, methyltransferase domain; Y, Y domain; PCP, papain-like cysteine protease domain; X, X or macro domain; Hel, helicase domain; RdRp, RNA-dependent RNA polymerase domain.
1These authors contributed equally to this article.