Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 19, Number 4—April 2013
Research

Occult Hepatitis B Virus Infection in Chacma Baboons, South Africa

Caroline Dickens, Michael C. Kew, Robert H. Purcell, and Anna KramvisComments to Author 
Author affiliations: University of the Witwatersrand, Johannesburg, South Africa (C. Dickens, M.C. Kew, A. Kramvis); Groote Schuur Hospital, Cape Town, South Africa (M.C. Kew); National Institutes of Health, Bethesda, Maryland, USA (R.H. Purcell)

Main Article

Figure 4

Nested PCR amplification of a subgenomic region of cDNA for hepatitis B virus, South Africa. Reverse transcribed, DNase I–treated cDNA products were amplified by PCR, and amplicons were resolved by electrophoresis on a 1% agarose gel containing ethidium bromide. Non–reverse transcribed samples (in which diethyl pyrocarbonate [DEPG]–treated water was added instead of enzyme during reverse transcription) were included as negative controls. Top panel: Lanes 1–7, nested PCR 1: 255F–759R; lanes 8–14,

Figure 4. . . Nested PCR amplification of a subgenomic region of cDNA for baboon hepatitis B virus, South Africa. Reverse transcribed, DNase I–treated cDNA products were amplified by PCR, and amplicons were resolved by electrophoresis on a 1% agarose gel containing ethidium bromide. Non–reverse transcribed samples (in which diethyl pyrocarbonate [DEPC]–treated water was added instead of enzyme during reverse transcription) were included as negative controls. Top panel: Nested PCR 1: 255F–759R; PCR 2: 459F–710R. Bottom panel, 550-bp region of glyceraldehyde-3-phosphate dehydrogenase gene amplified to assess quality of mRNA. Lanes 1 and 8, 100 mg of baboon liver tissue used for RNA extraction; lanes 2 and 9, RNA extraction negative control; lanes 3 and 10, 200 mg of baboon liver tissue used for RNA extraction; lanes 4 and 11, RNA extraction negative control; lane 5, DNase I treatment negative control in which DEPC-treated water was added instead of RNA; lane 6, reverse transcription negative control in which DEPC-treated water was added instead of cDNA; lanes 7 and 12, double-round nested PCR negative control containing best-quality water instead of cDNA; lane 13, single-round PCR negative control containing best-quality water instead of cDNA; lane 14, PCR-positive control containing DNA extracted from liver tissue of baboon 9732 as template.

Main Article

Page created: March 13, 2013
Page updated: March 13, 2013
Page reviewed: March 13, 2013
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external