Zichun Xiang, Zhengde Xie, Zhong Wang, Lili Ren, Yan Xiao, Linlin Li, Guy Vernet, Gláucia Paranhos-Baccalà, Kunling Shen, and Jianwei Wang
Figure. . . Phylogenetic tree of human enteroviruses (EVs) for nucleotide sequences of the viral protein (VP) 4/VP2 gene region (435 nt, corresponding to nt positions 654–1,088 of EV-C104 prototype strain CL-12310945 [EU840733]), People’s Republic of China, March 2007–February 2012. The tree was generated with 1,000 bootstrap replicates. Neighbor-joining analysis of targeted nucleotide sequence was performed by using the Kimura 2-parameter model with the Molecular Evolutionary Genetics Analysis software version 4.0 (www.megasoftware.net/). Each strain detected in this study is indicated by a black circle and a specific identification code (BCH/PUMCH), followed by the patient number. Enterovirus 68, cocksackievirus (CV) A2, and echovirus (E) 3 (GenBank accession nos. AY426531, AY421760, and AY302553) were used as outgroups. PV, poliovirus. EV-C denotes the EV species to which EV-C104 belongs. Scale bar indicates evolutionary distance.