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Volume 20, Number 1—January 2014
Research

Progenitor “Mycobacterium canettii” Clone Responsible for Lymph Node Tuberculosis Epidemic, Djibouti

Yann Blouin, Géraldine Cazajous, Céline Dehan, Charles Soler, Rithy Vong, Mohamed Osman Hassan, Yolande Hauck, Christian Boulais, Dina Andriamanantena, Christophe Martinaud, Émilie Martin, Christine Pourcel, and Gilles VergnaudComments to Author 
Author affiliations: Université Paris-Sud, Orsay, France (Y. Blouin, Y. Hauck, C. Pourcel, G. Vergnaud); Centre National de la Recherche Scientifique, Orsay (Y. Blouin, Y. Hauck, C. Pourcel, G. Vergnaud); Hôpital Militaire Bouffard, Djibouti, Republic of Djibouti (G. Cazajous, C. Dehan, C. Boulais); Hôpital d'Instruction des Armées Percy, Clamart, France (C. Soler, R. Vong, C. Martinaud); Hôpital Paul Faure, Djibouti (M. Osman Hassan); Hôpital d'Instruction des Armées Bégin, Saint-Mandé, France (D. Andriamanantena); Centre Hospitalier Lyon Sud, Lyon, France (E. Martin); Institut de Recherche Biomédicale des Armées, Brétigny, France (G. Vergnaud)

Main Article

Table 2

Sequence analysis of CRISPRs alleles of the Mycobacterium canettii isolates, Djibouti, 2010–2013*

Cluster Strain No. spacers Allele code† Alias‡ (accession no.)
A Percy3 and all other clone A strains 26 III-A-69@94 STB-D CIPT140060008 (NC_019950)
C Percy1004 12 III-A-69@74-95-96-89@91-94
C Percy32 30 III-A-69@74-95@98-75@91-94
C CIPT140010059 29 III-A-69@74-95@98-75@91-94 STB-A CIPT140010059 (NC_015848)
– Percy79 31 III-A-99@129
– Percy301 31 III-A-99@129
B Percy214 8 I-C-130@137 STB-H CIPT140070013
B Percy525 8 I-C-130@137
C Percy1101 14 I-C-130@143
– Percy25 7 I-C-131-132-144@148 STB-E CIPT140070002
– Percy65 27 (1 doublet) I-C-201@205-203-206@226 STB-J CIPT140070017 (NC_019952)
– Percy327 9 I-C-130-133@137-198@200 STB-L CIPT140070008 (NC_019965)
– Percy302 50; 53 I-C-149@191-178var-192@197;I-Cvar-337@389 STB-K CIPT140070010 (NC_019951)
– Percy89 83 I-E-228-230@232-88var-233@240-245@258-278@333 STB-G CIPT140070005
– Percy99b 58 I-E-227@232-88var-233
@280-334@336 STB-I CIPT140070007
– Percy157 52 I-E-227@232-88var-233@277

*CRISPR, clustered regularly interspaced short palindromic repeats.
†The CRISPR type is indicated as prefix (type III-A, I-C, I-Cvar, I-E). Allele codes refer to the spacers dictionary (Technical Appendix 2 Table 1). "69@94" indicates that all spacers from 69 to 94 are present. Spacers 1 to 68 have been previously reported in M. tuberculosis (9). Spacer numbering in "M. canettii" runs from 69 up to 389, i.e., the total number of spacers observed in M. canettii is 321.
‡Strains sequenced by (5) as draft or completed (European Nucleotide Archive accession no. indicated) genome 88var = TCCAGAGGTCGAAGTGATGTTCGGTGTTCTCCT.

Main Article

Page created: January 03, 2014
Page updated: January 03, 2014
Page reviewed: January 03, 2014
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