Figure 2. . . Phylogenetic analysis of the complete spike (S) gene of the fulminating disease of guinea fowl coronavirus (gfCoV, in boldface) in relation to turkey coronaviruses (TCoVs) at the nucleotide level. The tree was generated by using MEGA 5.05 (http://megasoftware.net/mega.php) by the neighbor-joining method, Kimura 2-parameter model, and pairwise deletion. Bootstrap values (1,000 replicates) >75 are indicated on the nodes. Only a partial S gene sequence (1,657 nt) was available for quail CoV/Italy/Elvia/2005. Scale bar indicates kimura distance.