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Volume 20, Number 1—January 2014
Dispatch

Avian Hepatitis E Virus in Chickens, Taiwan, 2013

Ingrid W.-Y. Hsu and Hsiang-Jung TsaiComments to Author 
Author affiliations: National Taiwan University, Taipei, Taiwan (I.W.-Y. Hsu, H.-J. Tsai); and Council of Agriculture, New Taipei City, Taiwan (H.-J. Tsai)

Main Article

Table 2

Primers used in the generation of the complete genome of the Taiwan avian hepatitis E virus strain*

Primer name† Primer sequence, 5′→3′ Direction
TWN6490–6470 CTAGAAGTCGGCGTGTCTCAG R
TWN6469–6448 GTGACTGGTCCTCAGGTGCTTG R
TWN6244–6270 CAGGAGTGGATCTATTTCCTTCAGAAC F
TWN6243–6262 CCAGGAGTGGATCTATTTCC F
TWN6215–6241 GATACTTCTATCAGTACAACAACACAC F
TWN5705–5686 CGCAGCAGCGTGGATGGTAG R
TWN5506–5526 GTTAAGGTGACTGCTCCGCAC F
TWN5043–5023 GTCCTGAAGTGGCATGAGCGG R
TWN4784–4763 CTTCCGGCTGGGAGCGTTTGGG R
TWN3421–3441 CCATTGTCGCCTGGCTGCACC F
TWN3227–3247 GACGGGTTATTGGATATACCG F
TWN2952–2932 GGGTTCGACTGCTTGGCCACC R
TWN2930–2909 GAGTAAACACAATTTTTTGGCC R
TWN2569–2588 CGGGGCTGTTGCGATTACGC F
TWN1657–1635 CATAATGTGCAACGATGGCGGCG R
TWN1607–1582 CAGCAAGCTCTTTAAGTGTGAGTAGC R
TWN1570–1546 GAGGTCAATCAAATTCTCAGTGCTG R
TWN1145–1123 CAGCAATGGCAACAGCCGTCAGC R
TWN1114–1091 CTCAGGCTGCCAACCCTCATTGGC R
TWN1048–1026 GTAGGTCAGCAAGCGCGAGCAGC R
TWN670–646 CTTGTCCGTTGTATTTACGGTATTG R
TWN607–584 CTCCTCTGGTAAGTGCAACACGAC R
TWN580–557 CAGTGTCCGCATATTATGGCGGGC R
HaHEV1–26‡ GCGGCCGCTCTAGCTGCAGCGAATAC F
HaHEV1410–1433‡ CATCCGTGCGGGTACTAAATCTGC F
Anchored-oligo (dT)18 primer NVTTTTTTTTTTTTTTTTTT NA
Oligo d(T)-anchor primer GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV NA

*The annealing temperature was set at 60°C for each primer combination. R, reverse; F, forward; NA, not applicable.
†Numbers following TWN in primer names is the nucleotide position extracted by the respective primers.
‡Primers were designed on the basis of the sequence from Hungary (GenBank accession no. JN997392).

Main Article

Page created: January 03, 2014
Page updated: January 03, 2014
Page reviewed: January 03, 2014
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