Volume 20, Number 10—October 2014
Research
Lyme Disease, Virginia, USA, 2000–2011
Figure 4
![Maximum-likelihood phylogenetic reconstruction of Ixodes scapularis lineages based on 16S rRNA gene sequences using Tamura 3-parameter model (35). All samples beginning with IS were collected during this study; reference sequence GenBank accession numbers are indicated, as were sampling locations (2-letter state abbreviation). The clade containing samples collected in GA, FL, NC, OK, and SC is known as the Southern clade (sensu Norris et al. [20]); the clade containing all samples from this stud](/eid/images/13-0782-F4.jpg)
Figure 4. Maximum-likelihood phylogenetic reconstruction of Ixodes scapularis lineages based on 16S rRNA gene sequences using Tamura 3-parameter model (35). All samples beginning with IS were collected during this study; reference sequence GenBank accession numbers are indicated, as were sampling locations (2-letter state abbreviation). The clade containing samples collected in GA, FL, NC, OK, and SC is known as the Southern clade (sensu Norris et al. [20]); the clade containing all samples from this study, indicated by the prefix IS, represents the American clade (more complete explanation of these terms is provided in the text). Bootstrap values at nodes are based on 500 replicates. Scale bar indicates nucleotide substitutions per site.
References
- Bacon RM, Kugeler KJ, Mead PS. Surveillance for LD—United States, 1992–2006. MMWR Surveill Summ. 2008;57:1–9 .PubMed
- Centers for Disease Control and Prevention. LD incidence rates by state, 2002–2011 [cited 2013 May 7]. http://www.cdc.gov/lyme/stats/chartstables/incidencebystate.html
- Goddard J. Ecological studies of adult Ixodes scapularis in central Mississippi: questing activity in relation to time of year, vegetation type, and meteorologic conditions. J Med Entomol. 1992;29:501–6 .PubMed
- Centers for Disease Control and Prevention. Incidence by state, 2005–2010 [cited 2012 Jul 1]. http://www.cdc.gov/lyme/stats/chartstables/incidencebystate.html
- Casteel M, Sonenshine D. Abundance of adult Ixodes scapularis and infection with Borrelia burgdorferi in eastern Virginia. Va J Sci. 1996;47:293–300.
- Sonenshine DE, Ratzlaff RE, Troyer J, Demmerle S, Demmerle ER, Austin WE, Borrelia burgdorferi in eastern Virginia: comparison between a coastal and inland locality. Am J Trop Med Hyg. 1995;53:123–33 .PubMed
- Amerasinghe FP, Breisch NL, Azad AF, Gimpel WF, Greko M, Neidhardt MK, Distribution, density and LD spirochete infection in Ixodes dammini (Acari: Ixodidae) on white-tailed deer in Maryland. J Med Entomol. 1992;29:54–61 .PubMed
- Amerasinghe FP, Breisch NL, Neidhardt K, Pagac B, Scott TW. Increasing density and Borrelia burgdorferi infection of deer-infesting Ixodes dammini (Acari:Ixodidae) in Maryland. J Med Entomol. 1993;30:858–64 .PubMed
- Qiu WG, Dykhuizen DE, Acosta MS, Luf BJ. Geographic uniformity of the LD spirochete (Borrelia burgdorferi) and its shared history with tick vector (Ixodes scapularis) in the northeastern United States. Genetics. 2002;160:833–49 .PubMed
- Norris DE, Klompen JSH, Keirans JE, Black WIC. Population genetics of Ixodes scapularis (Acari: Ixodidae) based on mitochondrial 16S and 12S genes. J Med Entomol. 1996;33:78–89 .PubMed
- Van Zee J, Black WC, Levin M, Goddard J, Smith J, Piesman J. High SNP density in the black-legged tick, Ixodes scapularis, the principal vector of LD spirochetes. Ticks Tick Borne Dis. 2013;4:63–71.
- Diuk-Wasser MA, Vourc’h G, Cislo P, Hoen AG, Melton F, Hamer SA, Field and climate-based model for predicting the density of host-seeking nymphal Ixodes scapularis, an important vector of tick-borne diseases agents in the eastern United States. Glob Ecol Biogeogr. 2010;19:504–14.
- Centers for Disease Control and Prevention. Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of LD. MMWR Morb Mortal Wkly Rep. 1995;44:590–1 .PubMed
- Engstrom SM, Shoop E, Johnson RC. Immunoblot interpretation criteria for serodiagnosis of early LD. J Clin Microbiol. 1995;33:419–27 .PubMed
- ESRI. ESRI demographic updates: 2012/2017, and ESRI white paper. ESRI. 2012; http://www.esri.com/library/whitepapers/pdfs/demographic-update-methodology-2012.pdf
- Sonenshine DE. Ticks of Virginia (Acari, Metastigmata). Insects of Virginia series, no. 13. Blacksburg (VA): Virginia Polytechnic Institute and State University Press. 1979. 35 pp.
- Trout RT, Steelman CD, Szalanski AL. Population genetics and phylogeography of Ixodes scapularis from canines and deer in Arkansas. Southwest Entomologist. 2009;34:273–87. DOI
- Tamura K. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C content biases. Mol Biol Evol. 1992;9:678–87 .PubMed
- Oliver JH Jr, Owsley MR, Hutcheson HJ, James AM, Chen C, Irby WS, Conspecificity of the ticks Ixodes scapularis and I. dammini (Acari: Ixodidae). J Med Entomol. 1993;30:54–63 .PubMed
- Spielman A, Levine JF, Wilson ML. Vectorial capacity of North American Ixodes ticks. Yale J Biol Med. 1984;57:507–13 .PubMed
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